Cabecera 2019 CBMSO CSIC UAM

Monday, 19th August 2019

Institutional statement on the use of research animals

The Center for Molecular Biology Severo Ochoa has joined the agreement on Openness on Animal Research, promoted from the Federation of Scientific Societies in Spain (COSCE), with the collaboration of the European Association of Research Animals (EARA), and launched on 20 September 2016.

We are convinced that animal experimentation plays a fundamental role in the discovery of the underlying biological mechanisms of disease, and in the developing of medical treatments. Without research in animals, we would not have most of the medicines, antibiotics, vaccines and surgical techniques that are applied nowadays in human and veterinary medicine.

An important part of the research undertaken at the CBMSO aims at contributing to the improvement of human health and wellbeing and is carried out thanks to the use of animals, in the following areas of research: Cell Biology and Immunology, Virology and Microbiology, Development and Regeneration, Molecular Neuropathology and Genome Dynamics and Function.

The welfare of animals used for research purposes is of paramount relevance for the CBMSO, and also the strict compliance and respect to the current legislation on the protection of animals used in research and for other scientific purposes, including education. Our aim is to achieve the highest standards in animal welfare, not only from the point of view of our moral responsibility on them, but also because we are convinced we could not achieve research excellence without proper animal welfare. Our experiments with animals follow the legal standard and are assessed by an Ethics Committee on Animal Experimentation that promotes the use of alternative methods, the reduction in the number of animals used and the refinement in the experimental protocols applied. Not a single research project requiring the use of animals could start without the appropriate and required Ethics assessment and the eventual authorization from the competent authorities.

The Center for Molecular Biology Severo Ochoa also ensures that the personnel involved in animal care and researchers do have the adequate education and training and the required professional skills, and that all resources are provided to properly keep research animals in terms of facilities, husbandry, wellbeing and veterinary care.

The Centro de Molecular Biología Severo Ochoa has participated in some initiatives that of transparency in animal experimentation as the production of the video-documentary "Inside the cell", the organization and presentation of specific seminars of training in the management of experimental animals, and the participation of researchers from the Center in several outreach programs for RTVE. Also, the summary of the projects that are carried out in the CBMSO that use animals and their purpose can be consulted by anyone interested in the website of the Spanish Ministry of Economy, Industry and Competitiveness.

 

International RTs Symposium 2017

RIO TINTO, FUNDAMENTAL AND APPLIED ASPECTS OF A TERRESTRIAL MARS ANALOGUE

Río Tinto (Iberian Pyrite Belt, SW Spain), is a 92 km long extreme acidic river, product of the metabolic activity of chemolithotrophic microorganisms thriving in the high concentration of metal sulfides of the Iberian Pyrite Belt and considered one of the best geochemical and mineralogical terrestrial analogues of the wet past of Mars.

An extensive microbial ecology study during the last thirty years has identified the most important metabolic activities operating in the water column, the sediments and the subsurface of this peculiar ecosystem. This three day transdisciplinary Symposium aims to gather the experience and knowledge of geologists, geomicrobiologists, astrobiologists, environmentalists and miners interested in the Tinto basin as well as other extreme environments. Through comparison and discussion we will improve our understanding of the role of chemolithotrophy in the origin of life along with the formation of ore deposits, the generation of extreme acidic conditions, the biological influence on dissolution and precipitation of minerals, its biotechnological use for metal extraction, recovery and bioremediation, the eukaryotic diversity associated to these environments and the astrobiological interest it has as a model system for past or present life on Mars.

Two field trips are being offered, one centered on the past and present day influence of microorganisms in the ore forming processes in the Iberian Pyrite Belt and the other on the geomicrobiological and environmental aspects of the Tinto basin.

The Symposium, organized by the Centro de Astrobiología (INTA-CSIC), the Centro de Biología Molecular Severo Ochoa (UAM-CSIC) and the Universidad Autónoma de Madrid (UAM) will take place June 5th to 7th, 2017, on the campus of the UAM, easy to reach from downtown Madrid. We would like to see you in Madrid for this important transdisciplinary event.

Ricardo Amils

Full Professor of Microbiology and Chairman of RTs

 

International RTs Symposium 2017

 

Descubierta una proteína que “debilita” la barrera interna de los vasos sanguíneos durante la inflamación

  • En enfermedades inflamatorias crónicas la permeabilidad de la barrera endotelial se altera y provoca que los vasos sanguíneos se puedan romper, con problemas coagulatorios y edemas
  • La expresión de esta proteína “debilitadora” se ha observado en el hígado y en lesiones intestinales de pacientes con enfermedad de Crohn
  • El equipo investigador ha sido financiado por el Ministerio de Economía y Competitividad y por el Instituto de Investigación Sanitaria de la Fundación Jiménez Díaz

 

Imagen del endotelio con la proteína RhoB (verde), citoesqueleto (rojo) y las uniones intracelulares (azul)

 

Cuando se produce una inflamación en un tejido, la capa interna o barrera endotelial de los vasos sanguíneos se reduce para permitir el paso de células inmunitarias hacia el foco de la inflamación. Pero si las inflamaciones se producen de forma crónica, como ocurre en varias enfermedades, la permeabilidad de esta barrera se altera de forma patológica y provoca que los vasos sanguíneos sean proclives a romperse. Por ello estas enfermedades están asociadas a problemas coagulatorios y edemas. Ahora un estudio internacional liderado por investigadores del Consejo Superior de Investigaciones Científicas (CSIC) ha identificado la proteína que "debilita" esta capa interna de los vasos sanguíneos durante la inflamación. El trabajo se publica en la revista Journal of Cell Biology.

"Se trata de la proteína RhoB, cuya expresión se incrementa cinco veces en respuesta a la citoquina inflamatoria TNF, una señal crucial en muchas patologías inflamatorias”, explica el doctor Jaime Millán, jefe del grupo de Biología Celular de la Inflamación en el Centro de Biología Molecular Severo Ochoa del CSIC, quien ha liderado el estudio. El equipo de investigadores ha combinado varias técnicas bioquímicas para rastrear e identificar proteínas cuya expresión se altera en respuesta a dicha citoquina. “Este aumento de la permeabilidad vascular es regulado por el endotelio, que forma una capa de células unidas entre sí por complejos de proteínas", señala.

"Hemos identificado una doble función para esta proteína", indica Millán. "Por un lado RhoB se coordina con otras dos proteínas muy similares de la misma familia, RhoA y RhoC, para regular el citoesqueleto y las uniones intercelulares del endotelio", explica el investigador.

"Sin embargo, RhoB juega un papel adicional en células en un ambiente inflamatorio que no tienen los otros miembros de la familia: cuando células endoteliales humanas modificadas para tener niveles bajos de RhoB se veían expuestas a estímulos coagulantes, que provocan contracción y permeabilidad vascular en una situación de inflamación, éstas eran capaces de volver a extender más rápidamente sus membranas tras la contracción, de modo que reformaban más eficientemente la barrera endotelial en comparación con las células normales", explica Millán. "Al contrario, las células endoteliales expresando mutantes de RhoB con una actividad exacerbada, permanecían permanentemente contraídas."

"Hemos encontrado que RhoB es una proteína de las vesículas de transporte que inhibe y retiene en el interior celular ciertas proteínas esenciales para que el borde de la célula se extienda tras la contracción y contacte de nuevo con las células vecinas para reparar la barrera endotelial", detalla el investigador.

"Estos mecanismos de extensión de membrana identificados durante la reparación de la barrera endotelial son muy similares a los que intervienen en las protrusiones que emiten las células individuales para moverse. Los comparten desde las amebas hasta las células tumorales, lo que nos recuerda que las barreras celulares de los organismos complejos provienen de agrupaciones ancestrales de células individuales de las que conservamos sus mecanismos básicos", argumenta.

El equipo de investigación está estudiando si estos mecanismos de recuperación de la función de barrera funcionan en otros tipos celulares, principalmente en el endotelio de córnea, que forma la barrera responsable de mantener la transparencia de este tejido y por tanto la visión.

El grupo de Biología Celular de la Inflamación del CBMSO está financiado por el Ministerio de Economía y Competitividad y por la Fundación Jiménez Díaz. En el trabajo han participado también investigadores del CSIC, la Universidad Autónoma de Madrid, King’s College de Londres, los Hospitales Universitarios de Oviedo y Salamanca y la Universidad de Cádiz.

 

Beatriz Marcos-Ramiro, Diego García-Weber, Susana Barroso, Jorge Feito, María C. Ortega, Eva Cernuda-Morollón, Natalia Reglero-Real, Laura Fernández-Martín, Maria C. Durán, Miguel A. Alonso, Isabel Correas, Susan Cox, Anne J. Ridley and Jaime Millán. RhoB controls endothelial barrier recovery by inhibiting Rac1 trafficking to the cell border. Journal of Cell Biology. May 2, 2016, 10.1083/jcb.201504038

 

CBMSO announces its participation in the European project METAFLUIDICS

The Horizon2020 project METAFLUIDICS “Advanced toolbox for rapid and cost-effective functional metagenomic screening -microbiology meets microfluidics” will kick-off in June 2016 and includes the participation of 5 companies, 4 universities and 4 research organizations from 6 different countries. In that project, a total amount of over € 8.8 million will be funded by the European Commission.

During its course, the METAFLUIDICS project will integrate a range of technologies into a platform designed to beat the odds of identifying hits in metagenomic libraries faster, more efficiently and by a wider user base. Exploration and exploitation of the metagenome will be made faster and more successful by (i) ultrahigh-throughput screening in picoliter droplets that dramatically lowers the cost per assay to well below 0.01 cents and allows throughput of 107 assays per hour; (ii) workflows that streamline and increase the yield of library construction and functional expression and (iii) workflows for efficient bioinformatic analysis of hits based on user-friendly software solutions for metagenome analysis. This platform will be used to identify enzymes for biosynthesis of therapeutic small molecules, for green bioenergy conversion, bioremediation, food chemistry and other industrial applications.

Dr. Aurelio Hidalgo coordinates this action with a budget allocation of over €1.1 million, in which UAM will carry out tasks related to developing different enrichment and screening assays for metagenomic libraries in microfluidic format.

 

Growth of thermophillic bacteria expressing a yellow fluorescent protein inside micrometric water-in-oil droplets

 

Convenio de Colaboración Fundación Severo Ochoa y la Fundación Isabel Gemio

Se ha firmado un convenio de colaboración entre la Fundación Severo Ochoa y la Fundación Isabel Gemio para promover la investigación de enfermedades metabólicas hereditarias. En la era de la medicina de precisión el diagnóstico genético se postula como un elemento básico para la aplicación de un tratamiento individualizado. Este proyecto, que se desarrollará en el Centro de Investigación y Diagnóstico de Enfermedades Metabólicas hereditarias (CEDEM) situado en la UNIVERSIDAD AUTONOMA DE MADRID y en el Centro de Biología Molecular Severo Ochoa, pretende mejorar el diagnóstico de enfermedades metabólicas genéticamente no diagnosticadas, en concreto las enfermedades mitocondriales y los defectos congénitos de glicosilación, como primera aproximación para explorar novedosas estrategias terapéuticas que podrán ser utilizadas para el tratamiento de estas y otras enfermedades minoritarias.

 

Convenio FSO y Fundación Isabel Gemio

 

Firma Convenio FSO y Fundación Isabel Gemio

 

 

Financiación de Proyectos

 

 

 

 

 

Galería Fotos

S.M. la Reina Letizia y S.M. la Reina Rania de Jordania visitan el Centro de Biología Molecular Severo Ochoa

20 de Noviembre de 2015

             
 

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Fotografías facilitadas por Oficina de Protocolo de la Casa Real.

 

 

Recent Publications

pdf flat small Astrocytic p38α MAPK drives NMDA receptor-dependent long-term depression and modulates long-term memory

pdf flat small A specific isoform of Pyd/ZO-1 mediates junctional remodeling and formation of slit diaphragms

pdf flat small Bacterial Ligase D preternary-precatalytic complex performs efficient abasic sites processing at double strand breaks during nonhomologous end joining

pdf flat small Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation

pdf flat small Insights into ligand binding by a viral tumor necrosis factor (TNF) decoy receptor yield a selective soluble human type 2 TNF receptor

pdf flat small How many cadherins do human endothelial cells express?

pdf flat small A Heterologous Cell Model for Studying the Role of T-Cell Intracellular Antigen 1 in Welander Distal Myopathy

pdf flat small Extracellular Vesicles in Herpes Viral Spread and Immune Evasion

pdf flat small Genomic assemblies of newly sequenced Trypanosoma cruzi strains reveal new genomic expansion and greater complexity

pdf flat small Tetraspanins, Another Piece in the HIV-1 Replication Puzzle

pdf flat small Different mitochondrial genetic defects exhibit the same protein signature of metabolism in skeletal muscle of PEO and MELAS patients: A role for oxidative stress

pdf flat small Dynamic regulation of NOTCH1 activation and Notch ligand expression in human thymus development

pdf flat small Antigen phagocytosis by B cells is required for a potent humoral response

pdf flat small Enhancement of collagen deposition and cross-linking by coupling lysyl oxidase with bone morphogenetic protein-1 and its application in tissue engineering

pdf flat small Dynamic regulation of NOTCH1 activation and Notch ligand expression in human thymus development

pdf flat small R-Ras1 and R-Ras2 Are Essential for Oligodendrocyte Differentiation and Survival for Correct Myelination in the Central Nervous System

pdf flat small The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model

pdf flat small Chemokines cooperate with TNF to provide protective anti-viral immunity and to enhance inflammation

pdf flat small Chromatin conformation regulates the coordination between DNA replication and transcription

pdf flat small The Zn-finger domain of human PrimPol is required to stabilize the initiating nucleotide during DNA priming

Buscador Publicaciones Digital CSIC:

Oferta de Laboratorio de Cultivos y Animalario NC3

El CBMSO ofrece un servicio de utilización de instalaciones de nivel de contención 3, ya que cuenta con un laboratorio de cultivos con nivel de bioseguridad 3 (NC3) y un laboratorio con nivel de bioseguridad 3 (NC3) en el animalario para la manipulación de virus humanos y virus de interés en sanidad animal y parásitos que requieren este nivel de contención, ambos conectados entre sí. Estas instalaciones autorizadas cumplen con los más exigentes requisitos marcados por la normativa vigente. Los diferentes sistemas que se han implementado, en el diseño y en las características constructivas, tienen como objetivo la contención de todos los flujos que se puedan realizar durante las operaciones y fundamentalmente a la entrada y salida de los laboratorios de personas, materiales y residuos, asegurando en todo momento la contención biológica, principalmente en el sistema de tratamiento de aire, con filtros específicos en la salida y de residuos, ya que disponen de un sistema de tratamiento de todos los residuos líquidos generados o biowaste. Cuentan con el equipamiento específico, como cabinas de seguridad biológica, racks ventilados, SAS de entrada y salida de muestras, sistemas de desinfección específicos (autoclaves y SAS de peróxido de hidrógeno), que garantizan el cumplimiento de todas las medidas de bioseguridad y biocontención.

Cada proyecto deberá ser previamente valorado y aprobado por las correspondientes comisiones del centro y su coste se establecerá según el protocolo de trabajo. Para formalizar la colaboración, la solicitud deberá ser remitida a: This email address is being protected from spambots. You need JavaScript enabled to view it.

2014-03-12Compoanuncio.Labo.NC3.V05

Oferta Laboratorio Seguridad P3

El CBMSO ofrece un servicio de utilización de instalaciones de nivel de contención 3 (NC3, P3) de cultivos celulares y animalario conectados entre sí. El coste de cada proyecto se establecerá según el protocolo de trabajo, que deberá ser previamente valorado y aprobado por las correspondientes comisiones del centro. Para formalizar la colaboración, el proyecto deberá ser dirigido con antelación a   This email address is being protected from spambots. You need JavaScript enabled to view it.

Cuenta con un laboratorio de cultivos con nivel de bioseguridad 3 ( NC3) y un laboratorio con nivel de bioseguridad 3 (NC3) en el animalario para la manipulación de virus humanos y virus de interés en sanidad animal y parásitos que requieren este nivel de contención. Estas instalaciones autorizadas cumplen con los más exigentes requisitos marcados por la normativa vigente. Los diferentes sistemas que se han implementado, en el diseño y en las características constructivas, tienen como objetivo la contención de todos los flujos que se puedan realizar du-rante las operaciones y fundamentalmente a la entrada y salida de los laboratorios de personas, materiales y residuos, asegurando en todo momento la contención biológica, principalmente en el sistema de tratamiento de aire, con filtros específicos en la salida y de residuos, ya que disponen de un sistema de tratamiento de todos los residuos líquidos generados o biowaste. Cuentan con el equipamiento específico, como cabinas de seguridad biológica racks ventilados, SAS de entrada y salida de muestras ,sistemas de desinfección específicos (autoclaves y SAS de peróxido de hidrógeno) ,que garantizan el cumplimiento de todas las medidas de bioseguridad y biocontención.

 

Cuarto de cultivos NC3:

2014-03-12  Compo anuncio Labo NC3 V01

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