Cabecera 2019 CBMSO CSIC UAM

Thursday, 24th October 2019
Homogenizador GEA Niro  

Fabricante: GEA Niro Soavi

Equipos: 1

Ubicación: Planta -1, 030

Características técnicas:

  • Presión de trabajo: 2000bares
  • Caudal de trabajo: 150 ml/minuto
Fermentador Biostat B Plus  

Fabricante: B. Braun Biotech

Equipos: 2

Ubicación: Planta -1, 030

Características técnicas:

  • Reactor agitado (STR) automático
  • Volumen de trabajo 4 litros
  • Control PDI "online" de: Temperatura, pH, pO2, agitación, sustrato y espumas
  • Control jerárquico de O2: agitación y flujo másico de aire
  • Software: MFCS/win3.0
Fermentador Biostat ED10  

Fabricante: B. Braun Biotech

Equipos: 1

Ubicación: Planta -1, 030

Características técnicas:

  • Reactor agitado (STR) automático
  • Volumen de trabajo 10 litros
  • Control PDI "online" de: Temperatura, pH, pO2, agitación, presión y espumas
  • Control jerárquico de O2: agitación, presión, estación de mezcla de gases
  • Posibilidad de trabajar como Airlift Loop Reactor (ALR)
  • Software : MFCS/3.0
Fermentador Biostat C Plus  

Fabricante: B. Braun Biotech

Equipos: 1

Ubicación: Planta -1, 030

Características técnicas:

  • Reactor agitado (STR) automático
  • Volumen de trabajo 30 litros
  • Control PDI "online" de: Temperatura, pH, agitación y espumas
  • Control de flujo másico de aire
  • Control jerárquico de oxígeno disuelto
  • Esterilizable "in situ"
  • Software: MFCS/win3.0
    FERMENTACIÓN
 

Responsable Científico:
José Berenguer
Responsable Servicio:
Dionisio Ureña

 

Fermentación

 
Aplicación Cepa Destacable Resistencia Proveedor
Clonajes rutinarios DH5 Buen rendimiento plásmido
Insertos estables
Plásmidos gran tamaño
  Invitrogen
XL1Blue MRF' Insertos estables
Permite preparaciones ssDNA
Clonaje DNA sin metilar
Tet Stratagene
Top 10 Buen rendimiento plásmido
Insertos estables
Str Invitrogen
Clonaje difícil Sure 2 Deficiente en los genes implicados en reordenación y delección de DNA
Mejora insertos DNA inestable
Tet/Kan Stratagene
HST08 Clonaje cDNA
Clonaje DNA metilado
Vectores hasta 10Kb
  Clontech
JM110 Deficiente en las metilasas Dam y Dcm Str Stratagene
DH110Bac Clonaje baculovirus
Genera bacmidos para transfectar células de insectos
Kan/Tet Invitrogen
XL10 Gold Aumenta la transformación de DNA de gran tamaño
Deficiente en endonucleasas y sistemas de restricción
Tet/Cam Stratagene
Able K Reduce el número de copias en vectores derivados de ColE1, aumentando la probabilidad de propagar clones tóxicos Kan/Tet Stratagene
JM101 Sistemas de restricción y recombinación intactos   NEB
DB3.1 Para propagar plásmidos que contienen el operón ccdB como Gateway® Str Invitrogen
Aplicación Cepa Destacable Resistencia Fuente
Expresión doble hibrido BTH101 Estudios de interacción proteína - proteína Str Euromedex
Expresión con promotor lac M15pREP4 Expresión proteínas con vectores pQE Kan QIAGEN
SG13009pREP4 Expresión proteínas con vectores pQE Kan QIAGEN
Expresión con promotor T7 BL21(DE3) Deficiente en las proteasas Lon y OmpT   Stratagene
BL21(DE3) pLysS pLys S no es un vector de clonaje. Suprime la expresión basal del promotor T7 por producción de lisozima Cam Stratagene
Tuner (DE3) Niveles de inducción regulables por concentración de IPTG   Novagen
C41(DE3) Expresión de proteínas toxicas y de membrana   Lucigen
C43(DE3) Expresión de proteínas toxicas y de membrana   Lucigen
Origami 2 (DE3) Mejora la formación de puentes disulfuro en el citoplasma Tet, Str Novagen
Lemo 21(DE3) Niveles de lisozima regulables por L-ramnosa Cam NEB
Rosetta-Gami 2(DE3) Combina la expresión de siete tRNAs raros en E.coli con la formación mejorada de puntes disulfuro Kan, Tet, Str, Cam Novagen
ArticExpress(DE3)RP Crece a baja temperatura para mejorar la solubilidad de las proteínas. Contiene Cpn60 y Cpn10 de Oleispira antarctica Tet, Gent, Str Agilent Technologies
Rosetta (DE3) pLysS Expresa seis tRNAs raros, facilita la expresión de codones raros en E.coli. Represión de la expresión basal de proteína por lisozima Cam Novagen
    FERMENTACIÓN
 

Responsable Científico:
José Berenguer
Responsable Servicio:
Dionisio Ureña

 

Fermentación

 

En el Servicio de Fermentación estamos implicados en la búsqueda de la mejor estrategia para la producción de biomasa y proteína. Asesoramos a los usuarios sobre las diferentes alternativas para optimizar la producción de proteína heteróloga y resolver determinados problemas de plegamiento y agregación. También proporcionamos asistencia técnica y desempeñamos nuestro trabajo en los siguientes entornos:

  • Bioprocesos para producción de cultivos microbianos en escala de laboratorio y piloto
  • Optimización de cultivos en batch, feedbatch y continuo
  • Expresión de proteína heteróloga en procariotas y eucariotas
  • Producción de antígenos
  • Desarrollo de medios de cultivo
  • Marcajes con isótopos estables

Servicio de Fermentación

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