Cabecera 2019 CBMSO CSIC UAM

Thursday, 24th October 2019

Criosubstitución Automática 550px

Técnicas:

  • Criosustitución de especímenes biológicos (deshidratación a baja temperatura, entre -80ºC y -90ºC)
  • Crioinclusión de especímenes biológicos con resinas acrílicas (Lowicryl)  y  polimerización con luz UV a baja temperatura

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 2018 10 16 Microscopio Transmision 03 550px

Características técnicas:

  • Cañón de emisión termoiónica: admite filamento de tungsteno y de LaB6
  • Voltaje de aceleración: hasta 120 keV
  • Lente objetivo: pieza polar tipo STEM (EM-10151ST12)
  • Resolución entre líneas: 0,2 nm
  • Goniómetro motorizado en 5 ejes
  • Portamuestras de alto ángulo de inclinación para uso en tomografía

    Línea1pxBorde

 Cámara CMOS Oneview (Gatan)

CamaraGatanV2

Características técnicas:

  • Sensor CMOS 4K x 4K (tamaño pixel 15 um2)
  • Acoplamiento del centelleador al CMOS por fibra óptica 1:1
  • Velocidad de captura: 25  fps a máxima resolución
  • Rango dinámico: 16Bit

 

 2008 04 24 Microscopia Rev2018 550px

Técnicas:

  • Obtención de criocortes (técnica de Tokuyasu) entre - 80ºC y -120ºC de células y tejidos incluidos en gelatina y crioprotegidos con sacarosa
 

 

 

 

 

 

 

 

 

 

 

 

 

 

Maite 2008 04 24 Microscopia 11 Rev2018 550px

Técnicas:

  • Obtención de cortes semifinos (1um grosor) y ultrafinos ( < 90 nm) de muestras incluidas en resinas, a temperatura ambiente

 

 

 

 

 

 

 

 

 

 

 

Criofractura y Réplicas 950px

Técnicas:

  • Criofractura y criosecado de muestras congeladas
  • Obtención de réplicas metálicas de la superficie de fractura
  • Sombreado rotatorio con Pt/C a bajo ángulo de ácidos nucléicos

 

 

 

 

 

 

 

 

 

2008 04 24 Microscopia 28 Rev2018 550px

Técnicas:

  • Evaporación de carbón sobre la película plástica soporte (colodión o formvar) de rejillas de microscopía electrónica
  • Ionización de rejillas para mejorar la adsorción de las muestras

 

 

 

 

 

 

 

 

 

 

 

Criofijación por Inmersión 550px

Técnicas:

  • Vitrificación de muestras particuladas (complejos macromoleculares, virus, etc) por inmersión en etano líquido (plunge freezing)
  • Vitrificación en propano o etano líquidos de células y tejidos fijados para su procesamiento mediante criosustitución o criofractura.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

·       Vitrificación en propano o etano líquidos de células y tejidos fijados para su procesamiento mediante criosustitución o criofractura.

2018 11 02 Microscopio Veterano 1010 550px

Características técnicas:

  • Cañón de emisión termoiónica: filamento de tungsteno
  • Voltaje de aceleración: hasta 100 KeV
  • Lente objetivo: alto contraste (pieza polar SAP-10B)
  • Resolución entre líneas: 0,2 nm

  Línea1pxBorde

Cámara CMOS TemCam F416 (TVIPS)

Camara xf416T

Características técnicas:

  • Sensor CMOS 4Kx4K (tamaño pixel 15,6 um2)
  • Acoplamiento del centelleador al CMOS por fibra óptica 1:1
  • Velocidad de captura: 1.0 fps a máxima resolución
  • Rango dinámico: 16 Bit

 

    MICROSCOPÍA ELECTRÓNICA
 

Responsable Científico:
Germán Andrés Hernández
Responsable Servicio:Mª Teresa Rejas Marco

 

Microscopía Electrónica

 

El Servicio de Microscopía Electrónica (SME) proporciona apoyo científico-técnico a los grupos de investigación interesados en emplear la microscopía electrónica para el análisis ultraestructural e inmunocitoquímico de complejos macromoleculares, virus, bacterias, células eucariotas y tejidos. Los métodos disponibles incluyen técnicas convencionales de procesamiento de muestras y criotécnicas como la criosustitución, la crioultramicrotomia y la criofractura.

Además el SME ofrece diferentes métodos de localización in situ de ácidos nucleicos y proteínas, incluyendo técnicas de microscopía correlativa óptica-electrónica (CLEM) para la observación in vivo de moléculas de interés y su posterior localización ultraestructural.

El SME dispone de dos microscopios electrónicos de transmisión, de 100 kV y 120kV, ambos equipados con cámaras fotográficas digitales (4K x 4K) de última generación y de un microscopio invertido de fluorescencia dedicado a técnicas CLEM. Además dispone del siguiente equipamiento para el procesamiento de muestras: equipo de criofijación por inmersión, equipo de criosustitución automática, ultramicrotomo convencional, crioultramicrotomo y unidad de criofractura.

 

    MICROSCOPÍA ELECTRÓNICA
 

Responsable Científico:
Germán Andrés Hernández
Responsable Servicio:
Mª Teresa Rejas Marco

 

Microscopía Electrónica

 
    MICROSCOPÍA ELECTRÓNICA
 

Responsable Científico:
Germán Andrés Hernández
Responsable Servicio:
Mª Teresa Rejas Marco

 

Microscopía Electrónica

 
 
 
 
 

NOTE! This site uses cookies and similar technologies.

If you not change browser settings, you agree to it. Learn more

I understand

COOKIES POLICY

What are cookies?

A cookie is a file that is downloaded to your computer when you access certain web pages. Cookies allow a web page, among other things, to store and retrieve information about the browsing habits of a user or their equipment and, depending on the information they contain and the way they use their equipment, they can be used to recognize the user.

Types of cookies

Classification of cookies is made according to a series of categories. However, it is necessary to take into account that the same cookie can be included in more than one category.

  1. Cookies according to the entity that manages them

    Depending on the entity that manages the computer or domain from which the cookies are sent and treat the data obtained, we can distinguish:

    • Own cookies: those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which the service requested by the user is provided.
    • Third party cookies: those that are sent to the user's terminal equipment from a computer or domain that is not managed by the publisher, but by another entity that processes the data obtained through the cookies. When cookies are installed from a computer or domain managed by the publisher itself, but the information collected through them is managed by a third party, they cannot be considered as own cookies.

  2. Cookies according to the period of time they remain activated

    Depending on the length of time that they remain activated in the terminal equipment, we can distinguish:

    • Session cookies: type of cookies designed to collect and store data while the user accesses a web page. They are usually used to store information that only is kept to provide the service requested by the user on a single occasion (e.g. a list of products purchased).
    • Persistent cookies: type of cookies in which the data is still stored in the terminal and can be accessed and processed during a period defined by the person responsible for the cookie, which can range from a few minutes to several years.

  3. Cookies according to their purpose

    Depending on the purpose for which the data obtained through cookies are processed, we can distinguish between:

    • Technical cookies: those that allow the user to navigate through a web page, platform or application and the use of different options or services that exist in it, such as controlling traffic and data communication, identifying the session, access to restricted access parts, remember the elements that make up an order, perform the purchase process of an order, make a registration or participation in an event, use security elements during navigation, store content for the broadcast videos or sound or share content through social networks.
    • Personalization cookies: those that allow the user to access the service with some predefined general characteristics based on a series of criteria in the user's terminal, such as the language, the type of browser through which the user accesses the service, the regional configuration from where you access the service, etc.
    • Analytical cookies: those that allow the person responsible for them to monitor and analyse the behaviour of the users of the websites to which they are linked. The information collected through this type of cookies is used in the measurement of the activity of the websites, applications or platforms, and for the elaboration of navigation profiles of the users of said sites, applications and platforms, in order to introduce improvements in the analysis of the data of use made by the users of the service.

Cookies used on our website

The CBMSO website uses Google Analytics. Google Analytics is a simple and easy to use tool that helps website owners to measure how users interact with the content of the site. You can consult more information about the cookies used by Google Analitycs in this link.

Acceptance of the Cookies Policy

The CBMSO assumes that you accept the use of cookies if you continue browsing, considering that it is a conscious and positive action from which the user's consent is inferred. In this regard, you are previously informed that such behaviour will be interpreted that you accept the installation and use of cookies.

Knowing this information, it is possible to carry out the following actions:

How to modify the configuration of cookies

Using your browser you can restrict, block or delete cookies from any web page. In each browser the process is different, here we show you links on this particular of the most used browsers: