Cabecera 2019 CBMSO CSIC UAM

Thursday, 24th October 2019

Overview

 

The formation of an adult organism is the result of the progressive and spatio-temporally controlled specification of groups of cells, which simultaneously undergo cell divisions and movements under the tight control of networks of intra-cellular signalling pathways and transcription factors. Understanding these events is of fundamental importance to unravel the basis of many human diseases and normal aging and to design strategies for tissue repair or regeneration.

 

 

Read more about the Dept. of Development and Regeneration...

 

Director of the Dept:  Paola Bovolenta

Group Leader 

   Research
Antonio Baonza    Control of cell proliferation and organ regeneration through intercellular signals
Paola Bovolenta    Morphogenesis and differentiation of vertebrate central nervous system
Ana Busturia    Drosophila Developmental Epigenetics
Sonsoles Campuzano   Regulation of gene expression during Drosophila development
José F. de Celis    Cell signalling during imaginal development in Drosophila
Carlos Estella   Gene expression control, patterning and growth during appendage development
Isabel Guerrero    Signalling mechanisms during development
Fernando J. Díaz-Benjumea   Cell fate specification in the development of the Drosophila central nervous system
Fernando Martín Belmonte   Epithelial cell polarity and cancer
David Míguez   Biophysics and Systems Biology
Ginés Morata    Genetic control of morphogenesis
Mar Ruiz-Gómez   Genetic and functional analysis of the renal filtration diaphragm in health and disease
Ernesto Sánchez-Herrero    Segmental specification and pattern formation in Drosophila

 

  Ad Honorem Professors
     -   
 Antonio García-Bellido  
 Juan Modolell
                      Project Leaders 

Natalia Azpiazu
(Ginés Morata lab.)

Florencia Cavodeassi
(Paola Bovolenta lab.)
Nicole Gorfinkiel
(Isabel Guerrero lab.)

Development and Regeneration

       Epigenetic Regulation of gene expression during Drosophila development

 

 

 Busturia group 400px

 


Ana María de Busturia

CSciStaff

CPublications

 

 

 

Research Summary:

Research in my laboratory is focused on the study of the epigenetic regulation of gene ex-pression mediated by the Polycomb (PcG) and trithorax (trxG) groups of proteins as well as by microRNAs. We use Drosophila as a model system to understand normal and pathologi-cal development.

Gene transcriptional states are established as either active or repressed depending on the cellular context, biological process or developmental time. Once established, they have to be faithfully maintained throughout proliferation in order to achieve normal development. PcG and trxG proteins control the transcriptional memory and they do so by compacting chromatin and by modificationof histones. Moreover, microRNAs are also post-transcriptional regulators that bind to their mRNAs targets usually resulting in gene silencing. We aim to understand how activation and repression affects the homeostasis of an organism by studying the function of the microRNAs and the relevance of the expression levels and/or activity of the PcG/trxG proteins in normal and pathological development. This research is done through the study of the function of these epigenetic regulators in the control of cell proliferation, apoptosis and innate immune response. Moreover, to gain insight into the mechanisms that provide the PcG/trxG with the characteristics of a dynamic, reversible and adaptable control system, we also study to what external cues and signals the microRNAs and the PcG/trxG system responds.

As the microRNAs and the PcG/trxG proteins are highly conserved throughout the animal kingdom, it is expected that our research should yield results that directly impact on the understanding of the function of these proteins in other organisms, including humans. Morever, deciphering the role(s) of miRNAs and PcG/trxG may lead to a more profound understanding of the mechanisms controlling the genesis and progression of human diseases

  Figure 300px
 

 

Dp53-induced proliferation is dependent on Notch levels of expres-sion. a-f wing imaginal discs of the geno-types indicated stained with TO-PRO-3. The proliferation of the wing imaginal cells is not affected by the absence of one dose of Notch gene (b) or by the presence of an extra-dose of Notch (c) when compared to wt (a). However, the absence of one dose of Notch (e) or the presence of an extra-dose of Notch dra-matically affect the proliferation induced by high levels of Dp53 (c).

 

 

 

   
   
   

 

Relevant Publications:

  • Fereres, S., Simón, R., Mohd-Sarip, A., Verrijzer, CP., Busturia, A (2014) dRYBP counteracts chromatin-de-pendent activation and repression of transcription. PLoS One Nov 21;9(11):e113255.
  • Aparicio, R, Simoes da Silva, C., Busturia A (2014) The microRNA mir-7 contributes to the control of Drosophila wing growth. Developmental Dynamics. Jan;244(1):21-30.
  • Simón, R., Aparicio, R., Houdsen, B., Bray, S., Busturia, A (2014) Drosophila p53 controls Notch expression and balances apoptosis and proliferation. Apoptosis19 (10) 1430-43.
  • Fereres,S., Simón, R,  Busturia, A (2013) A novel dRYBP-SCF complex functions to inhibit apoptosis in Drosophila. Apoptosis 18, (12) 1500 -1512.
  • Aparicio, R., Neyen,C., Lemaitre, B., Busturia, A. (2013) dRYBP contributes to the negative regulation of Drosophila IMD pathway. PLoS One Apr 15:8 (4): e62052.

 

Doctoral Theses:

  • Rocio Simón Sacristán (2013). PhD Thesis. Función de las proteínas Polycomb/trithorax y Dp53 en la regulación de la expression génica de Drosophila. PhD Thesis. Universidad Autónoma de Madrid, Spain. Directora: Ana Busturia
  • Sol Fereres Rapoport (2014). PhD Thesis. dRYBP transcription-dependent and transcription-independent functions in Drosophila melanogaster. Universidad Autónoma de Madrid, Spain. Directora: Ana Busturia

 

Development and Differentiation - Overview

Overview

The formation of an adult organism is the result of the progressive and spatio-temporally controlled specification of groups of cells, which simultaneously undergo cell divisions and movements under the tight control of networks of intra-cellular signalling pathways and transcription factors. Understanding these events is of fundamental importance to unravel the basis of many human diseases and normal aging and to design strategies for tissue repair or regeneration. Our department gathers research groups interested in uncovering the cellular and molecular basis of different aspects of embryonic development and its implications in human health by using a variety of model systems, including snail, Drosophila, teleost fishes, chicken and mouse.

Our department comprises a total of seven teen staff scientists and two emeritus professors, grouped in twelve research teams. There are also four tenure-track researchers with independent projects. Our department research activities are structured around four major and highly interactive lines: a) Genetic and epigenetic control of developmental gene expression; b) Morphogenesis and cell-to-cell communication; c) Organ formation and regeneration; d) Understanding pathological processes through development.

Our department maintains active and fruitful collaborations with other national and international centers. Research activities in our department are supported by common technological platforms and facilities. Besides contributing to the Center Seminar Series, our department organizes weekly meetings, where student and postdocs have the opportunity to discuss their recent results and a bi-monthly seminar series, "New Frontiers in Developmental Biology", designed as a forum to debate current trends in the field. Members of our department also help to coordinate a discussion group to foster the exchange of ideas and collaborations among scientists in the Madrid area interested in keeping up with new technologies and achievements related to the zebrafish as a model system.

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