Thursday, 14th December 2017
    MICROSCOPÍA ÓPTICA Y CONFOCAL
 

Coordinador Científico:
Fco. Javier Díez-Guerra
Responsable Técnico:
Ángeles Muñoz

 

Microscopía Óptica y Confocal

 

SMOC

 

Comunidad de Madrid

 

ÚLTIMAS NOTICIAS

  • 07-12-2017: LSM510 Vertical reparado. Se ha cambiado el escáner X y el láser de Argón (línea 488).
  • 17-11-2017: Disponible de nuevo eescáner resonante del confocal Nikon A1R.
  • 16-11-2017: Apertura   confocal LSM800 a partir del lunes 20. Para realizar las reservas y organizar las sesiones de formación y asistencia, dirigirse al SMOC (lab 310).
  • 09-02-2017 Láser Maitai del Multifotón fuera de servicio. El equipo se puede utilizar como confocal.
 

ENLACES - REACTIVOS Y FLUORÓFOROS - MEDICIÓN DE CALCIO Y OTROS IONES

 

 

  • Intracellular pH mapping with SNARF-1 and confocal microscopy. (1993). Micron. Volume 24, Issue 6.
  • Rudolf, et al. (2003). Looking forward to seeing calcium. Nature Reviews Mol. Cell Biol. 4 , 579-586
  • Bunt and Wouters. (2004). Visualization of molecular activities inside living cells with fluorescent labels. Int.Rev.Cytology. 237, 205-277.
  • Takatsuka, et al. (2005). A novel Ca+2 indicator protein using FRET and calpain-sensitive linker. Biochem. Biophys. Res. Comm. 336, 316-323.
  • Kotlikoff, M. (2006). Genetically encoded Ca2+
  • O'Connor and Silver. (2007) Ratio Imaging: Practical Considerations for Measuring Intracellular Ca2+ and pH in Living Cells. Methods in Cell Biology. 81, 415-433.
  • Hires, et al. (2008). Reporting neural activity with genetically encoded calcium indicators. Brain Cell Biol. 36, 69–86.
  • Optical Methods in Calcium Signaling. (2008). Methods. Volume 46, Issue 3.
  • Barreto-Chang and Dolmetsch. (2009). Calcium Imaging of Cortical Neurons using Fura-2 AM. Journal of visualized experiments.
  • Benink , et al. (2009) Direct pH measurements by using subcellular targeting of 5(and 6-) carboxyseminaphthorhodafluor in mammalian cells. BioTechniques. 47, 769–774.
  • T, Balla. (2009). Green light to illuminate signal transduction events. Trends in Cell Biology. 19, 575-586.
  • Horikawa, et al. (2010). Spontaneous network activity visualized by ultrasensitive Ca2+ indicators, yellow Cameleon-Nano. Nature Methods. 7, 729–732.
  • Calcium in living cells. (2010). Methods in Cell Biology. 99, 2-316.
  • ZHao, et al. (2011). An Expanded Palette of Genetically Encoded Ca2+ Indicators. Science. 333, 1888-1891.
  • Methods in Enzymology. 2012. Volume 505, Pages 2-523.
  • Methods in Enzymology. 2012. Volume 506, Pages 2-490.
  • Chen, et al. (2013). Ultrasensitive fluorescent proteins for imaging neuronal activity. Nature. 499, 295-300.
  • Rodriguez-García, et al. (2014). GAP, an aequorin-based fluorescent indicator for imaging Ca2+ in organelles. PNAS. 111, 2584-2589.
  • Wilms and Häusser. (2014). Twitching towards the ideal calcium sensor. Nature Methods. 11, 139-140.