[et_pb_section fb_built=”1″ _builder_version=”4.25.0″ _module_preset=”default” background_color=”#34609B” background_image=”https:\/\/www.cbm.uam.es\/wp-content\/uploads\/2024\/06\/Captura-de-Pantalla-2024-06-13-a-las-17.46.16.png” background_position=”bottom_center” background_vertical_offset=”22%” min_height=”352px” height=”294px” da_disable_devices=”off|off|off” global_colors_info=”{}” da_is_popup=”off” da_exit_intent=”off” da_has_close=”on” da_alt_close=”off” da_dark_close=”off” da_not_modal=”on” da_is_singular=”off” da_with_loader=”off” da_has_shadow=”on”][et_pb_row column_structure=”2_5,3_5″ use_custom_gutter=”on” gutter_width=”4″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” custom_margin=”61px||||false|false” custom_padding=”||0px|||” global_colors_info=”{}”][et_pb_column type=”2_5″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”rgba(52,96,155,0.83)” custom_padding=”50px||50px|50px|false|false” border_radii=”on|15px|15px|15px|15px” global_colors_info=”{}”][et_pb_text _builder_version=”4.25.0″ _module_preset=”default” text_text_color=”#FFFFFF” custom_margin=”0px||10px||false|false” custom_padding=”0px||0px||false|false” global_colors_info=”{}”]<\/p>\n
Scientific facilities<\/p>\n
[\/et_pb_text][et_pb_heading title=”Drosophila transgenesis” _builder_version=”4.25.0″ _module_preset=”default” title_font=”Montserrat||||||||” title_text_color=”#FFFFFF” title_font_size=”38px” title_line_height=”1.1em” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][et_pb_column type=”3_5″ _builder_version=”4.23.4″ _module_preset=”default” custom_padding=”157px||||false|false” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.23.4″ _module_preset=”default” background_enable_image=”off” background_size=”custom” background_image_height=”84%” background_position=”bottom_right” da_disable_devices=”off|off|off” global_colors_info=”{}” da_is_popup=”off” da_exit_intent=”off” da_has_close=”on” da_alt_close=”off” da_dark_close=”off” da_not_modal=”on” da_is_singular=”off” da_with_loader=”off” da_has_shadow=”on”][et_pb_row _builder_version=”4.23.4″ _module_preset=”default” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.23.4″ _module_preset=”default” custom_margin=”30px||||false|false” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”rgba(204,209,209,0.18)” custom_padding=”15px|15px|15px|15px|true|true” global_colors_info=”{}”][et_pb_blurb title=”Facility Head” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Mar Casado” image=”\/imgs\/310.jpg” image_icon_width=”200px” module_class=”roundedImg” _builder_version=”4.27.0″ _module_preset=”default” header_text_align=”center” header_font_size=”14px” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Nuria Esteban” image=”\/imgs\/1784.jpg” image_icon_width=”200px” module_class=”roundedImg” _builder_version=”4.27.0″ _module_preset=”default” header_text_align=”center” header_font_size=”14px” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Scientific Lead” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Carlos Estella” image=”\/imgs\/1733.jpg” image_icon_width=”200px” module_class=”roundedImg” _builder_version=”4.27.0″ _module_preset=”default” header_text_align=”center” header_font_size=”14px” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Contact details” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” body_font_size=”11px” body_line_height=”1.6em” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”91 196 46 10″ use_icon=”on” font_icon=”||fa||900″ icon_color=”#008C93″ icon_placement=”left” image_icon_width=”16px” _builder_version=”4.25.0″ _module_preset=”default” header_font_size=”14px” image_icon_custom_margin=”|-5px||50px|false|false” image_icon_custom_padding=”||||false|false” custom_margin=”-11px||5px||false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”transgenesisdro (at) cbm.csic.es” use_icon=”on” font_icon=”||divi||400″ icon_color=”#008C93″ icon_placement=”left” image_icon_width=”16px” _builder_version=”4.25.0″ _module_preset=”default” header_font_size=”14px” image_icon_custom_margin=”|-5px||50px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Fees” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” body_font_size=”11px” body_line_height=”1.6em” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_icon font_icon=”||fa||400″ icon_color=”#13B5BD” icon_width=”24px” url=”\/wp-content\/uploads\/2024\/06\/Fees-transgenesis-dosophila.pdf” url_new_window=”on” _builder_version=”4.25.0″ _module_preset=”default” custom_margin=”-21px|||-167px|false|false” custom_padding=”0px||||false|false” global_colors_info=”{}”][\/et_pb_icon][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.23.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
The Drosophila transgenesis facility was created in 2008 with the aim of providing technical support to the research groups of the CBM, the CSIC and other \u00a0national and international institutes, through the generation of genetically modified Drosophila<\/em> lines.<\/p>\n Our main qualities are:<\/p>\n – Efficiency: With a success rate of more than 98% in obtaining transgenic stocks.<\/p>\n – Speed: We send the injected larvae within 5-9 days after receiving the samples<\/p>\n – Low cost: Very competitive prices compared to other national and international services.<\/p>\n [\/et_pb_text][et_pb_accordion open_toggle_text_color=”#34609B” closed_toggle_text_color=”#34609B” closed_toggle_background_color=”rgba(175,218,249,0.39)” toggle_icon=”G||divi||400″ icon_color=”#008C93″ _builder_version=”4.27.5″ _module_preset=”default” animation_style=”slide” animation_direction=”bottom” hover_enabled=”0″ box_shadow_style=”preset2″ box_shadow_spread=”-10px” global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”Classic transgenesis mediated by the P transposable element” open=”on” _builder_version=”4.25.0″ _module_preset=”default” transform_scale=”-2%|-2%” custom_css_main_element=”display: none;” border_width_all=”0px” border_color_all=”RGBA(255,255,255,0)” border_style_all=”none” global_colors_info=”{}”]<\/p>\n The transgenes insert at random positions in the genome occurs. The plasmid must contain the inverted repeats of the P element as well as the mini-white gene to identify the positives.<\/p>\n We require 10 ug<\/strong> of DNA \u00a0in a volumen of 10ul<\/strong> for each construct injected. It is not necessary for the user to provide the DNA helper.<\/p>\n Strains available in the service:<\/strong><\/p>\n – w, yw<\/strong><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Classic transgenesis mediated by the P transposable element” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”22px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n The transgenes insert at random positions in the genome occurs. The plasmid must contain the inverted repeats of the P element as well as the mini-white gene to identify the positives.<\/p>\n We require 10 ug<\/strong> of DNA \u00a0in a volumen of 10ul<\/strong> for each construct injected. It is not necessary for the user to provide the DNA helper.<\/p>\n Strains available in the service:<\/strong><\/p>\n – w, yw<\/strong><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Transgenesis mediated by PhiC31 integrase” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n Transgene insert at well-defined receptor sites in the genome, \u00a0containing attP sequences.<\/p>\n Transgenes should be cloned into vectors containing attB sites and the mini-white gene to identify the positives.<\/p>\n DNA preparations should be of high quality and at a minimum concentration of 300 ng\/ul<\/strong> in a minimum volume of 20 ul.<\/strong><\/p>\n Strains available from the service:<\/strong><\/p>\n –2L<\/strong> y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP’}ZH-22A B#24481<\/strong><\/p>\n –2R<\/strong> y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP}ZH-51D B#24483<\/strong><\/p>\n –3L<\/strong> y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP’}ZH-68E B#24485<\/strong><\/p>\n –3R<\/strong> y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP}ZH-86Fb B#24749<\/strong><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Genome editing by CRISPR\/Cas9 technology” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n We can inject mixtures of nucleic acids containing gRNAs, plasmids encoding gRNAs and ssDNA or plasmid templates for CRISPR\/Cas9-assisted genomic editing. The strain we used expresses Cas9 in the germline under the control of the nanos promoter.<\/p>\n The service cannot provide technical support regarding the experimental design or the different possible technical approaches.<\/p>\n The user must provide us with the nucleic acid mixture ready for injection. The service recommends: 3ug guideline RNA and 10ug donno<\/strong>r in 20ul<\/strong> volume.<\/p>\n Strains available from the service:<\/strong><\/p>\n -25C<\/strong> y[1] v[1] P{y[+t7.7]=nos-phiC31int.NLS}X;P{y[+t7.7]=CaryP}attP40 B#25709<\/strong><\/p>\n -68A <\/strong>y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31int.NLS}X; P{y[+t7.7]=CaryP}attP2 B#25710<\/strong><\/p>\n –Noscas9<\/strong>: y[1] M{w[+mC]=nos-Cas9.P}ZH-2A w[*]\u00a0 B#54591<\/strong><\/p>\n –Vascas9 RFP-:<\/strong>y[1]M{GFP[E.3xP3]=vas-Cas9.RFP-}ZH-2Aw[1118] B#55821<\/strong><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Husbandry of user provided stocks” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n \u00a0<\/strong>When users need to inject samples into specific strains from their laboratories, our facility will charge for the husbandry of these strains<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Instructions” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.27.5″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n DNA PREPARATION<\/strong><\/p>\n We recommend purifying the DNA using the Qiagen kit or similar. The concentration and purity of the DNA must be analyzed before shipping.<\/p>\n <\/p>\n TIMELINE<\/strong><\/p>\n For the generation of transgenes using the P element or PhiC31 integrase, we inject 300 to 400 embryos per plasmid. In the case of the CRISPR\/Cas9 method, we inject 500 to 600 embryos per plasmid.<\/p>\n The surviving larvae (between 75 and 150) are sent to the user 3 to 4 days after injection.<\/p>\n Users will be responsible for analyzing the transgenic lines in their laboratories. If no transformants are obtained, we will repeat the injection once, with priority and at no additional cost<\/strong>.<\/p>\n <\/p>\n SHIPPING<\/strong><\/p>\n The shipping costs for the larvae after injection are borne by the user. There are several transport companies available.<\/p>\n <\/p>\n SAMPLE SHIPPING INSTRUCTIONS<\/strong><\/p>\n Samples should be sent to the following address:<\/p>\n Mar Casado\/Nuria Esteban To access this service, users must complete the following forms<\/strong>. If this is your first time using our service, you must also sign the legal agreement<\/strong>.<\/strong><\/p>\n <\/p>\n DOCUMENTS<\/span><\/span><\/span><\/span><\/strong><\/p>\n
Severo Ochoa Molecular Biology Center
C\/Nicol\u00e1s Cabrera 1
28049, Madrid
Phone: +34-911964610<\/p>\n