[et_pb_section fb_built=”1″ _builder_version=”4.25.0″ _module_preset=”default” background_color=”#34609B” background_image=”https:\/\/www.cbm.uam.es\/wp-content\/uploads\/2024\/06\/cell-culture2-scaled.jpeg” background_position=”bottom_center” background_vertical_offset=”22%” min_height=”352px” height=”294px” da_disable_devices=”off|off|off” global_colors_info=”{}” da_is_popup=”off” da_exit_intent=”off” da_has_close=”on” da_alt_close=”off” da_dark_close=”off” da_not_modal=”on” da_is_singular=”off” da_with_loader=”off” da_has_shadow=”on”][et_pb_row column_structure=”2_5,3_5″ use_custom_gutter=”on” gutter_width=”4″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” custom_margin=”61px||||false|false” custom_padding=”||0px|||” global_colors_info=”{}”][et_pb_column type=”2_5″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”rgba(52,96,155,0.83)” custom_padding=”50px||50px|50px|false|false” border_radii=”on|15px|15px|15px|15px” global_colors_info=”{}”][et_pb_text _builder_version=”4.25.0″ _module_preset=”default” text_text_color=”#FFFFFF” custom_margin=”0px||10px||false|false” custom_padding=”0px||0px||false|false” global_colors_info=”{}”]<\/p>\n
Scientific facilities<\/p>\n
[\/et_pb_text][et_pb_heading title=”Cell and tissue culture models and techniques” _builder_version=”4.27.0″ _module_preset=”default” title_font=”Montserrat||||||||” title_text_color=”#FFFFFF” title_font_size=”36px” title_line_height=”1.1em” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][et_pb_column type=”3_5″ _builder_version=”4.23.4″ _module_preset=”default” custom_padding=”157px||||false|false” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.25.0″ _module_preset=”default” background_enable_image=”off” background_size=”custom” background_image_height=”84%” background_position=”bottom_right” da_disable_devices=”off|off|off” global_colors_info=”{}” da_is_popup=”off” da_exit_intent=”off” da_has_close=”on” da_alt_close=”off” da_dark_close=”off” da_not_modal=”on” da_is_singular=”off” da_with_loader=”off” da_has_shadow=”on”][et_pb_row _builder_version=”4.23.4″ _module_preset=”default” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.23.4″ _module_preset=”default” custom_margin=”30px||||false|false” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”rgba(204,209,209,0.18)” custom_padding=”15px|15px|15px|15px|true|true” global_colors_info=”{}”][et_pb_blurb title=”Facility Head” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” body_font_size=”11px” body_line_height=”1.6em” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”M\u00aa del Rosario Francisco Velilla” image=”https:\/\/www.cbm.uam.es\/imgs\/492.jpg” image_icon_width=”200px” module_class=”roundedImg” _builder_version=”4.27.4″ _module_preset=”default” header_text_align=”center” header_font_size=”14px” hover_enabled=”0″ global_colors_info=”{}” sticky_enabled=”0″][\/et_pb_blurb][et_pb_blurb title=”Contact details” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” body_font_size=”11px” body_line_height=”1.6em” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”91 196 46 65″ use_icon=”on” font_icon=”||fa||900″ icon_color=”#008C93″ icon_placement=”left” image_icon_width=”16px” _builder_version=”4.25.0″ _module_preset=”default” header_font_size=”14px” image_icon_custom_margin=”|-5px||50px|false|false” image_icon_custom_padding=”||||false|false” custom_margin=”-11px||5px||false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”peticion-medios (at) cbm.csic.es” use_icon=”on” font_icon=”||divi||400″ icon_color=”#008C93″ icon_placement=”left” image_icon_width=”16px” _builder_version=”4.25.0″ _module_preset=”default” header_font_size=”14px” image_icon_custom_margin=”|-5px||50px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Fees” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.27.4″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” body_font_size=”11px” body_line_height=”1.6em” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_icon font_icon=”||fa||400″ icon_color=”#13B5BD” icon_width=”24px” url=”\/wp-content\/uploads\/2025\/10\/Tarifas-cultivos-2025-26.pdf” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-21px|||-167px|false|false” custom_padding=”0px||||false|false” global_colors_info=”{}”][\/et_pb_icon][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.23.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.0″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n
The Cell and tissue culture facility offers the following services: [\/et_pb_text][et_pb_accordion open_toggle_text_color=”#34609B” closed_toggle_text_color=”#34609B” closed_toggle_background_color=”rgba(175,218,249,0.39)” toggle_icon=”G||divi||400″ icon_color=”#008C93″ _builder_version=”4.27.4″ _module_preset=”default” animation_style=”slide” animation_direction=”bottom” box_shadow_style=”preset2″ box_shadow_spread=”-10px” global_colors_info=”{}”][et_pb_accordion_item title=”Management of the cell and tissue culture laboratories” open=”on” _builder_version=”4.25.0″ _module_preset=”default” transform_scale=”-2%|-2%” custom_css_main_element=”display: none;” border_width_all=”0px” border_color_all=”RGBA(255,255,255,0)” border_style_all=”none” global_colors_info=”{}”]<\/p>\n The CBM has 6 biosafety level 2 laboratories for the use of cell cultures, with a total of 91 booths, distributed on floors 1, 2, 3, 4 and -1, with the same characteristics on floors 1 to 4. On the 1st floor there is a P2 laboratory for microbiology and on floor -1 the P2 laboratory is dedicated to work with viruses.<\/p>\n In addition, the CBM has a P3 laboratory that is used exclusively for experiments involving human and\/or animal cell cultures, human and\/or animal viruses, and parasites requiring biosafety level 3.<\/p>\n In these laboratories the service staff is responsible for:<\/p>\n Management of purchase of products necessary for the preparation of media for cell culture. <\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Management of the cell and tissue culture laboratories” toggle_icon=”||divi||400″ use_icon_font_size=”on” icon_font_size=”22px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n The CBM has 6 biosafety level 2 laboratories for the use of cell cultures, with a total of 91 booths, distributed on floors 1, 2, 3, 4 and -1, with the same characteristics on floors 1 to 4. On the 1st floor there is a P2 laboratory for microbiology and on floor -1 the P2 laboratory is dedicated to work with viruses.<\/p>\n In addition, the CBM has a P3 laboratory that is used exclusively for experiments involving human and\/or animal cell cultures, human and\/or animal viruses, and parasites requiring biosafety level 3.<\/p>\n In these laboratories the service staff is responsible for:<\/p>\n Management of purchase of products necessary for the preparation of media for cell culture. <\/span><\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Mycoplasma detection” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n Mycoplasma detection is carried out in cell lines and culture media by means of a colorimetric assay, based on the ability to recognize \u201cTOLL-LIKE-RECEPTOR 2\u201d mycoplasmas and to induce the activation of NF-kB and other transcription factors. In the presence of mycoplasmas TLR2 expressed on the surface of HEK-blue cells, activates these transcription factors which, in turn, induce the secretion of alkaline phosphatase, a protein easily detectable by the blue staining of the medium.<\/p>\n The assay will be performed periodically, informing users at least 5 days in advance, so that they can collect the samples to be tested and leave them in the place marked for this purpose by the service.<\/p>\n For the elimination of mycoplasmas in contaminated cultures, the service offers antibiotic aliquots and the protocol to follow.<\/p>\n [\/et_pb_accordion_item][et_pb_accordion_item title=”Washing and sterilization” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n <\/strong><\/p>\n General use material in the different laboratories and services of the CBM<\/strong><\/p>\n The Material Washing Service provides support to the Research Groups, as well as to other Services of the Center that require it.<\/p>\n The staff of the Service is in charge of the collection, cleaning and sterilization of the laboratory material and its orderly storage, leaving it available for collection by the users.<\/p>\n They are also in charge of the sterilization of all the media, buffers, solutions etc. Both those prepared in the Service and those prepared in the laboratories and require sterilization.<\/p>\n Location: Floor 2, Laboratory 210<\/p>\n Material for use in P2 culture laboratories<\/strong><\/p>\n The personnel of the Service are in charge of the collection of material from the culture laboratories and the deactivation of different residues, as well as the cleaning and sterilization of the laboratory material and its orderly storage, leaving it available for collection by the users. [\/et_pb_accordion_item][et_pb_accordion_item title=”Preparation of media and solutions” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n The media laboratory, where different media, buffer solutions, etc. are prepared, offers the user a permanent stock, with the possibility of placing orders.<\/p>\n Preparation of media and solutions for cells of animal origin<\/strong><\/p>\n A permanent stock of the following media and solutions is offered: Preparation of culture media for microorganisms<\/strong><\/p>\n Liquid media are prepared following the corresponding protocols according to demand and sterilized by heat in a 121\u00baC liquid cycle for 20 minutes. A permanent stock of the following media and solutions is available: Preparation of solutions and buffers<\/strong><\/p>\n They are prepared following the corresponding protocol (Molecular Cloning appendix, etc.) with controls on pH and physical characteristics. They are mostly sterilized by steam autoclaving or by filtration with a 0.22 \u00b5m membrane in a sterile cabinet.<\/p>\n A permanent stock of the following media and solutions is available: Filtration by 0.22 \u03bcm, in sterile laminar flow cabinet.
<\/strong><\/span><\/p>\n
Management of the purchase of the necessary equipment.
Preparation and sterilization of media and solutions used for the different work in cultures.
Control of the proper functioning and preventive maintenance of the equipment in the general culture room (incubators, cabinets, pipettors, etc.).
Control of the general cleanliness of the laboratories.
General maintenance of the laboratories (cleaning of equipment, removal of plastic and glass waste, replacement of material).
Elaboration of cleaning and maintenance protocols.
Elaboration of rules of use of the facility.
Control of invoicing per user.
Detection of mycoplasmas in the cultures of users who request this service.<\/p>\n
Management of the purchase of the necessary equipment.
Preparation and sterilization of media and solutions used for the different work in cultures.
Control of the proper functioning and preventive maintenance of the equipment in the general culture room (incubators, cabinets, pipettors, etc.).
Control of the general cleanliness of the laboratories.
General maintenance of the laboratories (cleaning of equipment, removal of plastic and glass waste, replacement of material).
Elaboration of cleaning and maintenance protocols.
Elaboration of rules of use of the facility.
Control of invoicing per user.
Detection of mycoplasmas in the cultures of users who request this service.<\/p>\n
Verification of the operation of the autoclaves by means of periodic controls (Vacuum Test, Bowie & Dick Test, and Biological Test).<\/p>\n
MEM 1X
RPMI
RPMI + hepes
PBS 1X
PBS 10X
PBS 1X compl.
BSS
DMEM 1X
DMEM 2X
TC-100
Antimycotic
Glutamine
EDTA
Gentamicin
M. antibiotics
AANE 100X
Trypsin EDTA
Sodium pyruvate
Trypsin
Special media
Agar1.4% Agar
Agar 1%
Neutral red 1,4+ Agar
M 3
A.C.D
DEAE-dextran
lysis buffer
HBSS
These media and solutions are prepared under maximum safety conditions, being carried out in a sterile laminar flow cabinet and filtered with 0.22 \u00b5m membranes. Subsequently, a sterility test is performed.
Location: Floor 2, Laboratory 211 and Floor -1, Laboratory 032.<\/p>\n
Solid media. Prepared with agar and heat sterilized in autoclave in liquid cycle 121\u00baC, 20 minutes.<\/p>\n
LB
LB+agar
Plates with LB+agar medium
Plates with LB+agar+antibiotic medium
2x YT medium
Location: Floor 1, Lab 123<\/p>\n
ECL (developer solution)
TBE 10X
TRIS GLYCINE 10X
TBS 10X
PBS 10X
TAE 50X
Mg Cl2 1M
K Ac 5M
SSC 20X
TRIS 1.5M pH 8.8
TRIS 1M pH 6.8
TRIS 1M pH 7.5
TRIS 1M pH 8
TRIS 1M pH 8.8
SDS 10% SDS
SDS 20% SDS
EDTA 0.5 M pH 8
Na Cl 5M
Na Ac 3M pH 5.2
Monosodium Phosphate 1M
Disodium Phosphate 1M
RIPA
PB 0,2 N Ph:7,4
Location: Floor 2, Laboratory 211
Serum treatment<\/p>\n
Sterility test.<\/p>\n