{"id":283532,"date":"2024-09-20T12:06:49","date_gmt":"2024-09-20T10:06:49","guid":{"rendered":"https:\/\/dhcp205002.cbm.uam.es\/?page_id=283532"},"modified":"2025-07-07T16:36:58","modified_gmt":"2025-07-07T14:36:58","slug":"microscopia-electronica","status":"publish","type":"page","link":"https:\/\/www.cbm.uam.es\/index.php\/servicios\/microscopia-electronica\/?lang=es","title":{"rendered":"Microscop\u00eda electr\u00f3nica"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.25.0″ _module_preset=”default” background_color=”#34609B” background_image=”https:\/\/www.cbm.uam.es\/wp-content\/uploads\/2024\/06\/Captura-de-Pantalla-2024-06-14-a-las-14.59.19-scaled.jpg” background_position=”bottom_center” background_vertical_offset=”22%” min_height=”352px” height=”294px” da_disable_devices=”off|off|off” global_colors_info=”{}” da_is_popup=”off” da_exit_intent=”off” da_has_close=”on” da_alt_close=”off” da_dark_close=”off” da_not_modal=”on” da_is_singular=”off” da_with_loader=”off” da_has_shadow=”on”][et_pb_row column_structure=”2_5,3_5″ use_custom_gutter=”on” gutter_width=”4″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”RGBA(255,255,255,0)” custom_margin=”61px||||false|false” custom_padding=”||0px|||” global_colors_info=”{}”][et_pb_column type=”2_5″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”rgba(52,96,155,0.83)” custom_padding=”50px||50px|50px|false|false” border_radii=”on|15px|15px|15px|15px” global_colors_info=”{}”][et_pb_text _builder_version=”4.25.0″ _module_preset=”default” text_text_color=”#FFFFFF” custom_margin=”0px||10px||false|false” custom_padding=”0px||0px||false|false” global_colors_info=”{}”]<\/p>\n

Servicios cient\u00edficos<\/p>\n

[\/et_pb_text][et_pb_heading title=”Microscop\u00eda electr\u00f3nica” _builder_version=”4.25.0″ _module_preset=”default” title_font=”Montserrat||||||||” title_text_color=”#FFFFFF” title_font_size=”43px” title_line_height=”1.1em” global_colors_info=”{}”][\/et_pb_heading][\/et_pb_column][et_pb_column type=”3_5″ _builder_version=”4.23.4″ _module_preset=”default” custom_padding=”157px||||false|false” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.23.4″ _module_preset=”default” background_enable_image=”off” background_size=”custom” background_image_height=”84%” background_position=”bottom_right” da_disable_devices=”off|off|off” global_colors_info=”{}” da_is_popup=”off” da_exit_intent=”off” da_has_close=”on” da_alt_close=”off” da_dark_close=”off” da_not_modal=”on” da_is_singular=”off” da_with_loader=”off” da_has_shadow=”on”][et_pb_row _builder_version=”4.23.4″ _module_preset=”default” custom_margin=”||0px||false|false” custom_padding=”||0px||false|false” global_colors_info=”{}”][et_pb_column type=”4_4″ _builder_version=”4.23.4″ _module_preset=”default” global_colors_info=”{}”][\/et_pb_column][\/et_pb_row][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.23.4″ _module_preset=”default” custom_margin=”30px||||false|false” custom_padding=”0px||||false|false” global_colors_info=”{}”][et_pb_column type=”1_3″ _builder_version=”4.23.4″ _module_preset=”default” background_color=”rgba(204,209,209,0.18)” custom_padding=”15px|15px|15px|15px|true|true” global_colors_info=”{}”][et_pb_blurb title=”Responsable del servicio” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Martin Christoph Sachse” image=”https:\/\/www.cbm.uam.es\/imgs\/6062.jpg” image_icon_width=”200px” module_class=”roundedImg” _builder_version=”4.27.4″ _module_preset=”default” header_text_align=”center” header_font_size=”14px” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Contacto” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.25.0″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” body_font_size=”11px” body_line_height=”1.6em” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”91 196 45 32″ use_icon=”on” font_icon=”||fa||900″ icon_color=”#008C93″ icon_placement=”left” image_icon_width=”16px” _builder_version=”4.27.4″ _module_preset=”default” header_font_size=”14px” image_icon_custom_margin=”|-5px||50px|false|false” image_icon_custom_padding=”||||false|false” custom_margin=”-11px||5px||false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”sme (at) cbm.csic.es” use_icon=”on” font_icon=”||divi||400″ icon_color=”#008C93″ icon_placement=”left” image_icon_width=”16px” _builder_version=”4.25.0″ _module_preset=”default” header_font_size=”14px” image_icon_custom_margin=”|-5px||50px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_blurb title=”Tarifas” use_icon=”on” font_icon=”W||divi||400″ icon_color=”#13B5BD” icon_placement=”left” image_icon_width=”25px” _builder_version=”4.27.4″ _module_preset=”default” header_text_align=”left” header_text_color=”#34609B” body_font_size=”11px” body_line_height=”1.6em” image_icon_custom_margin=”-3px|-1px|||false|false” image_icon_custom_padding=”|0px|||false|false” custom_margin=”|-1px||0px|false|false” global_colors_info=”{}”][\/et_pb_blurb][et_pb_icon font_icon=”||fa||400″ icon_color=”#13B5BD” icon_width=”24px” url=”https:\/\/www.cbm.uam.es\/wp-content\/uploads\/2025\/05\/Tarifas_SME_2025.pdf” url_new_window=”on” _builder_version=”4.27.4″ _module_preset=”default” custom_margin=”-21px|||-167px|false|false” custom_padding=”0px||||false|false” global_colors_info=”{}”][\/et_pb_icon][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.23.4″ _module_preset=”default” global_colors_info=”{}”][et_pb_text _builder_version=”4.27.0″ _module_preset=”default” global_colors_info=”{}”]<\/p>\n

El servicio de Microscop\u00eda electr\u00f3nica proporciona apoyo cient\u00edfico y t\u00e9cnico a los grupos de investigaci\u00f3n, tanto internos como externos al CBM, interesados en utilizar la microscop\u00eda electr\u00f3nica de transmisi\u00f3n para el an\u00e1lisis ultraestructural e inmunocitoqu\u00edmico de muestras biol\u00f3gicas.<\/p>\n

Podemos estudiar complejos macromoleculares, virus, bacterias, c\u00e9lulas eucariotas y tejidos. Los m\u00e9todos disponibles incluyen t\u00e9cnicas convencionales de procesamiento a temperatura ambiente y criot\u00e9cnicas como la criosustituci\u00f3n, la crioultramicrotom\u00eda y la criofractura. <\/p>\n

[\/et_pb_text][et_pb_accordion open_toggle_text_color=”#34609B” closed_toggle_text_color=”#34609B” closed_toggle_background_color=”rgba(175,218,249,0.39)” toggle_icon=”G||divi||400″ icon_color=”#008C93″ _builder_version=”4.27.4″ _module_preset=”default” animation_style=”slide” animation_direction=”bottom” hover_enabled=”0″ box_shadow_style=”preset2″ box_shadow_spread=”-10px” global_colors_info=”{}” sticky_enabled=”0″][et_pb_accordion_item title=”No hace nada!” open=”on” _builder_version=”4.27.4″ _module_preset=”default” transform_scale=”-2%|-2%” custom_css_main_element=”display: none;” border_width_all=”0px” border_color_all=”RGBA(255,255,255,0)” border_style_all=”none” global_colors_info=”{}”]<\/p>\n

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– Microscopio electr\u00f3nico de transmisi\u00f3n JEM1010 (Jeol, 1992)
– Ca\u00f1\u00f3n de emisi\u00f3n termoi\u00f3nica: filamento de tungsteno.
– Voltaje de aceleraci\u00f3n de hasta 100 kV.
– C\u00e1mara digital bajo columna CMOS TemCam F416 (TVIPS)<\/p>\n

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Microscopio electr\u00f3nico de transmisi\u00f3n JEM1400 Flash (Jeol, 2017)
– Ca\u00f1\u00f3n de emisi\u00f3n termoi\u00f3nica: filamento de LaB6.
– Voltaje de aceleraci\u00f3n hasta 120 kV.
– C\u00e1mara digital bajo columna CMOS de lectura r\u00e1pida OneView (Gatan).<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=”Microscopios electr\u00f3nicos de transmisi\u00f3n” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”22px” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n

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Microscopio electr\u00f3nico de transmisi\u00f3n JEM1010 (Jeol,1992)
– Ca\u00f1\u00f3n de emisi\u00f3n termoi\u00f3nica: filamento de tungsteno
– Voltaje de aceleraci\u00f3n hasta 100 kV.
– C\u00e1mara digital bajo columna CMOS TemCam F416 (TVIPS)<\/p>\n

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Microscopio electr\u00f3nico de transmisi\u00f3n JEM1400 Flash (Jeol, 2017)
– Ca\u00f1\u00f3n de emisi\u00f3n termoi\u00f3nica: filamento de LaB6.
– Voltaje de aceleraci\u00f3n hasta 120 kV.
– C\u00e1mara digital bajo columna CMOS de lectura r\u00e1pida OneView (Gatan).<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=”Equipos de tratamiento de muestras en fr\u00edo” toggle_icon=”||fa||400″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n

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Criofijaci\u00f3n por inmersi\u00f3n en l\u00edquido criog\u00e9nico ( \u201cplunge freezing\u201d ), equipo KF80 (Reichert-Jung, Leica,1992)
– Vitrificaci\u00f3n en etano l\u00edquido, de muestras particuladas (macromol\u00e9culas, virus…) adsorbidas a rejillas.
– Congelaci\u00f3n ultrar\u00e1pida de c\u00e9lulas o tejidos fijados qu\u00edmicamente y crioprotegidos para su procesamiento por criosustituci\u00f3n o criofractura.<\/p>\n

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Criofijaci\u00f3n por alta presi\u00f3n (HPF), equipo LEICA EM ICE (2023).<\/p>\n

– Vitrificaci\u00f3n de c\u00e9lulas, tejidos y peque\u00f1os organismos en condiciones nativas para su procesamiento por criosustituci\u00f3n o criofractura.<\/p>\n

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Criosustituci\u00f3n autom\u00e1tica, equipo LEICA EM AFS2 (2023).
– Deshidrataci\u00f3n a bajas temperaturas, -90 \u00baC, -80 \u00baC (criosustituci\u00f3n), crioinclusi\u00f3n en resinas Lowicryl y polimerizaci\u00f3n con luz UV a bajas temperaturas.
– Criosustituci\u00f3n previa a la inclusi\u00f3n en resina Epoxi a 4\u00baC- temperatura ambiente para an\u00e1lisis morfol\u00f3gicos.<\/p>\n

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Criofractura \/ criosecado, equipo BAF060 (Leica, 2007)
– Criofractura de c\u00e9lulas y tejidos y obtenci\u00f3n de r\u00e9plicas met\u00e1licas de la superficie fracturada.
– Sombreado rotatorio con Pt\/C de \u00e1cidos nucleicos y virus.<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=”Ultramicrotomos” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n

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Ultramicrotomo ULTRACUT E (Reichert-Jung, Leica)
– Obtenci\u00f3n de cortes semifinos, 1micra<\/span>, y ultrafinos, 60nm – 90nm a temperatura ambiente de muestras incluidas en resinas.<\/p>\n

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Ultramicrotomo UCT (Leica)
– Obtenci\u00f3n de cortes semifinos, 1micra<\/span>, y ultrafinos, 60nm – 90nm a temperatura ambiente de muestras incluidas en resinas.
– Obtenci\u00f3n de criosecciones a baja temperatura , – 80 \u00baC a -120\u00baC para inmunomarcaje (t\u00e9cnica de Tokuyasu).<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=”EVAPORADOR LEICA EM AC600 (2023)” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.27.4″ _module_preset=”default” hover_enabled=”0″ global_colors_info=”{}” open=”off” sticky_enabled=”0″]<\/p>\n

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– Recubrimiento de rejillas con carb\u00f3n evaporado.
– Ionizaci\u00f3n de rejillas mediante \u201cGlow discharge\u201d<\/p>\n

[\/et_pb_accordion_item][et_pb_accordion_item title=”T\u00e9cnicas” toggle_icon=”||fa||900″ use_icon_font_size=”on” icon_font_size=”20px” _builder_version=”4.25.0″ _module_preset=”default” global_colors_info=”{}” open=”off”]<\/p>\n

\u2013 Tinci\u00f3n negativa de complejos macromoleculares y virus. Fotot\u00e9cnica 1
\u2013 An\u00e1lisis de nanopart\u00edculas.
\u2013 Incrustaci\u00f3n convencional de muestras biol\u00f3gicas en resinas y ultramicrotom\u00eda. Foto t\u00e9cnica 2
\u2013 Criofijaci\u00f3n por inmersi\u00f3n en etano l\u00edquido y alta presi\u00f3n.
\u2013 Criosustituci\u00f3n e inclusi\u00f3n a baja temperatura. Foto t\u00e9cnica 3
\u2013 Inmunomicroscop\u00eda electr\u00f3nica con conjugados de oro coloidal en secciones de resina. Foto t\u00e9cnica 4
\u2013 Inmunomicroscop\u00eda electr\u00f3nica con conjugados de oro coloidal en criosecciones (t\u00e9cnica Tokuyasu). Foto t\u00e9cnica 5
\u2013 Criofractura, criosecci\u00f3n y obtenci\u00f3n de r\u00e9plicas met\u00e1licas. Foto t\u00e9cnica 6
\u2013 Microscop\u00eda electr\u00f3nica de \u00e1cidos nucleicos. Foto t\u00e9cnica 7
\u2013 Microscop\u00eda \u00f3ptica-electr\u00f3nica correlativa (CLEM). <\/p>\n

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