Overexpression models obtained by pronuclear microinjection of transgenes provided by the requesting users. The service provides 2-3 animals carrying the construct, but does not guarantee transmission to offspring or expression of the transgene. C57BL/6J or B6CBAF1 hybrid background is used. Other genetic backgrounds are possible upon request.

The service performs the search for vectors and ES lines available in international repositories for the different mouse genes. In case of vectors, it can perform electroporation in ES lines available to the service (G4 or R1). In case of cells, the service receives the cells, expands and genotypes them before injecting them into 8-cell stage mouse embryos. The service provides 2-3 high contribution chimeras, although it does not guarantee germline transmission.

In this type of models the support of the service covers from the design of guides to the generation of the animals carrying the desired modification. KO models are generated by electroporation, as are point mutations and small insertions. In case of larger inserts or for the generation of conditional animals, 1-cell phase microinjection is used. The service performs founder identification.

These are obtained from embryos of genetically altered lines provided by the applicant, by in vitro culture under conditions that maintain pluripotency. The service provides 1-2 vials of frozen cells once generated and if required, specific culture medium.

The performance of this service covers from the location of lines in the different international repositories, to the design of primers for genotyping, long PCR and Sanger sequencing or crossbreeding schemes for the generation of the desired genotype with the smallest possible number of animals, also advising on the necessary controls for the experiments.

The facility advises on the process of obtaining authorization for the creation of genetically altered lines. It also organizes and participates in different courses related to the management of genetically altered models and provides practical training in the procurement, culture and transfer of embryos.