Scientific facilities

Protein biotechnology

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Facility Head

María Gimeno

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Scientific Lead

Aurelio Hidalgo

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Contact details

91 196 44 81

prot_biotech (at) cbm.csic.es

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Fees

Traditionally dedicated to biomass production at fermenter scale, the facility is now evolving to meet the growing scientific demand for high-quality recombinant proteins. With a renewed focus on protein expression and purification, we aim to support research groups by providing tailored solutions and expert guidance throughout the entire bioprocess.

Core Services:

    • Microbial culture production at laboratory and pilot scale
    • Optimization of batch, fed-batch, and continuous fermentation processes
    • Heterologous protein expression in prokaryotic and yeast systems
    • Development of customized culture media
    • Protein purification 
    • E. coli repository and plasmid bank for gene cloning and protein expression
    • DNA molecular weight markers
Fermenters

Four fermenters are available for producing biomass and protein expression, with different working volumes:

1 x Biostat C Plus Fermenter (30 L)

1 x Biostat C15 fermenter (15 L)

2 x  Biostat B Plus fermenters – 4 L

 

Technical characteristics:

  • Automatic stirred reactors (STR).
  • Autoclavable or sterilisation-in-place depending on the model.
  •  Precise control of key parameters: pH, dissolved oxygen (DO), temperature and foam.
  • Compatible with microbial and cell culture applications.
  • Efficient agitation system with a wide speed range and low maintenance requirements.
  • Automated feeding options.
  • DCU controller with an intuitive graphical interface for process monitoring.
  • Software: Sartorius BioPAT MCFS.
Fermenters

Four fermenters are available for producing biomass and protein expression, with different working volumes:

1 x Biostat C Plus Fermenter (30 L)

1 x Biostat C15 fermenter (15 L)

2 x  Biostat B Plus fermenters – 4 L

 Technical characteristics:

  • Automatic stirred reactors (STR).
  • Autoclavable or sterilisation-in-place depending on the model.
  •  Precise control of key parameters: pH, dissolved oxygen (DO), temperature and foam.
  • Compatible with microbial and cell culture applications.
  • Efficient agitation system with a wide speed range and low maintenance requirements.
  • Automated feeding options.
  • DCU controller with an intuitive graphical interface for process monitoring.
  • Software: Sartorius BioPAT MCFS.

Biostat B plus

Biostat C

Biostat B Plus
Other equipment
  • Panda Plus 2000 Homogenizer (GEA Niro Soavi)
  • Centrifuges RC3 y RC5 (Sorvall) and continuous flux centrifuge (Westfalia)
  • Tangential filtration equipment (Vivascience)
  • Shaker incubators: Multitron standard (Infors HT), Innova 4080 and 4000 (New Brunswich Scientific)

Staff

María Isabel Carrascal Blanco

Lab.: 030 Ext.: 4481
mbcarrascal(at)cbm.csic.es

María Gimeno Pérez

Lab.: 030 Ext.: 4481
mgimeno(at)cbm.csic.es