Scientific facilities
Proteomics
Facility Head
Esperanza Morato López
Scientific Lead
María Yáñez Mo
Contact details
91 585 45 40 / 46 95
proteomica.servicio (at) cnb.csic.es
Fees
The Proteomics Facility offers advanced proteomics analytical services to the CBM scientific community, and also to any researcher from other public research centers, universities, hospitals and companies. As a support service for research activity, we provide personalized scientific/technical support to each project, which may include the stages before and after analysis: experimental design, projects review, data analysis and advice/support on the biological interpretation of generated data.
Our laboratory has been part of ProteoRed, a Proteomics Platform whose main objective is to integrate and coordinate the activities of the Spanish Proteomics Services as well as to evaluate the quality of the services offered (participating in the human proteome sequencing project (SpHP)). We are also members of the Spanish Proteomics Society (SEProt) and the European Proteomics Association (EuPA).
Since September 2024, the Proteomics Facility is a share centralized unit between the CBM and the CNB, and it is also integrated in the International Excellence Campus UAM+CSIC. The rates applied to CBM and CNB researches are those corresponding to the internal category.
Equipment
The platform has been significantly upgraded with a new lab and three new state-of-the art instruments, two Thermo Orbitrap Exploris 240 and one Orbitrap Eclipse Trybrid, equipped with FAIMS ion mobility modules, three nano HPLC, two Ultimate 3000, and one Vanquish Neo; and three analytical HPLCs: Ultimate 3000, Knauer, and Jasco, respectively. We also have an Opentrons T2 and an Accelerome (Thermo) platform for automatic sample preparation and an Intavis Multipep peptide synthesizer. Finally, the laboratory has licenses for data processing software: Mascot 2.8, Proteome Discoverer 3.1, Peaks 12.5 and Spectronaut 19.8, in addition to other open-source resources.
Equipment
The platform has been significantly upgraded with a new lab and three new state-of-the art instruments, two Thermo Orbitrap Exploris 240 and one Orbitrap Eclipse Trybrid, equipped with FAIMS ion mobility modules, three nano HPLC, two Ultimate 3000, and one Vanquish Neo; and three analytical HPLCs: Ultimate 3000, Knauer, and Jasco, respectively. We also have an Opentrons T2 and an Accelerome (Thermo) platform for automatic sample preparation and an Intavis Multipep peptide synthesizer. Finally, the laboratory has licenses for data processing software: Mascot 2.8, Proteome Discoverer 3.1, Peaks 12.5 and Spectronaut 19.8, in addition to other open-source resources.
Services
- Consulting and experimental design
- Characterization of pure molecules of peptide nature
- Characterization of interactomes
- Qualitative (identification) and quantitative analysis of proteomes (with isobaric or label free labeling)
- Selective protein quantification by multiple reaction monitoring (S/MRM-MS)
- Mass identification and characterization of post-translational modifications
- Peptide synthesis and design of membrane-bound peptide arrays
- Gluten analysis using ELISA, PCR and mass spectrometry
Instructions
Before requesting the Proteomics Platform services, it will be necessary to consult with the staff the experimental design in order to be able to effectively address the analyzes to be carried out and to be able to prepare a detailed budget
- You will be contacted through the Facility website:
https://proteo.cnb.csic.es/proteomica/
Staff
Esperanza Morato López
Lab.: B1-CNB Ext.: 915854540/4695
emorato(at)cbm.csic.es
Carlos García García
Lab.: B1-CNB Ext.: 915854540/4695
cgarcia(at)cbm.csic.es
Tamara Rosell García
Lab.: B1-CNB Ext.: 915854540/4695
trosell(at)cbm.csic.es
Alberto Rodríguez Izquierdo
Lab.: B1-CNB Ext.: 915854540/4695
alberto.rodriguez(at)cbm.csic.es