– Transmission electron microscope JEM1010 (Jeol,1992) Photo 1
– Thermionic emission gun: tungsten filament.
– Accelerating voltage up to 100kV.
– Digital camera under CMOS column TemCam F416 (TVIPS)
– JEM1400 Flash transmission electron microscope (Jeol, 2017) Fot2
– Thermionic emission gun: LaB6 filament.
– Accelerating voltage up to 120kV.
– OneView fast readout CMOS digital camera under column (Gatan).

• Cryofixation by immersion in cryogenic liquid (“plunge freezing” ), KF80 equipment (Reichert-Jung, Leica, 1992) Photo 3
  – Vitrification of particulate samples (macromolecules, viruses…) adsorbed on grids in liquid ethane.
  – Cryofixation of chemically fixed and cryoprotected cells or tissues for processing by cryosubstitution or cryofracturing.
• High pressure cryofixation (HPF), LEICA EM ICE (2023). Photo 4
  – Vitrification of cells, tissues and small organisms under native conditions for processing by cryosubstitution or cryofracturing.
• Automatic cryosubstitution, LEICA EM AFS2 (2023). Photo 5
  – Dehydration at low temperatures, -90 °C, -80 °C, cryoinclusion in Lowicryl resins and polymerization with UV light at low temperatures.
• Cryofracture/ Freeze-etching, BAF060 equipment (Leica, 2007) Photo 6
  – Cryofracture of cells and tissues and obtaining metallic replicas of the fractured surface.
  – Pt/C rotary shading of nucleic acids and viruses.

– Ultramicrotome ULTRACUT E (Reichert-Jung, Leica). Photo 7
– Obtaining semi-thin, 1µm, and ultrathin, 50nm – 90nm slices at room temperature of samples embedded in resins.
– Ultramicrotome with cryo camera, UCT (Leica). Photo 8
– Obtaining semifine, 1µm, and ultrafine, 50nm – 90nm slices at room temperature of samples included in resins.
– Obtaining cryosections at low temperature, – 80 ºC to -120ºC for immunolabeling, Tokuyasu technique.

Photo 9
– Coating of grids with evaporated carbon.
– Ionization of grids by “Glow discharge”.

– Negative staining of macromolecular complexes and viruses. Photo technique 1
– Analysis of nanoparticles.
– Conventional embedding of biological samples in resins and ultramicrotomy. Technical photo 2
– Cryofixation by immersion in liquid ethane and high pressure.
– Cryosubstitution and inclusion at low temperature. Technical photo 3
– Electron immunomicroscopy with colloidal gold conjugates in resin sections. Technical photo 4
– Electron immunomicroscopy with colloidal gold conjugates in cryosections (Tokuyasu technique). Technical photo 5
– Cryofracture, cryosectioning and obtaining metal replicas. Technical photo 6
– Electron microscopy of nucleic acids. Technical photo 7
– Correlative optical-electron microscopy (CLEM).