Foot-and-mouth disease virus (FMDV) is one of the major concerns for animal health. It is also an interesting model system for understanding the interactions of a highly variable virus and its natural hosts and the implications of these interactions on disease control. We are working in the development of new FMDV peptide marker vaccines that can induce protective humoral and cellular immune responses, using pig and cattle, important domestics hosts, as animal models. We have also analyzed the functional role of FMDV proteins on the viral particle stability and internalization, the replication cycle and the mechanisms mediating the pathogenesis of FMDV and other related viruses causing vesicular diseases, such as swine vesicular disease virus (SVDV), and vesicular stomatitis virus (VSV). Special attention has been paid to the functional implications of nonstructural proteins in virus virulence and host range. The role of different cellular lipids in the multiplication of these and other viruses such as West Nile virus (WNV), responsible for an important zoonosis, have also been addressed. As part of these studies, we have continued collaborating in the characterization of the inhibitory effect of valproic acid and other antiviral compounds targeting cellular metabolism such as lauryl gallate on the multiplication of FMDV and of other enveloped viruses, like African swine fever virus (ASFV) and type I herpesvirus.