CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOACaptura de pantalla 2022 09 14 a las 10.27.10    

Chromosome replication and genome stability

Research summary:

The maintenance of genome integrity during chromosome replication and the fidelity of DNA synthesis are essential for the correct transmission of genetic information in every cell division cycle. Inevitably, chromosome replication is threatened by DNA damage, which is a potential source of errors and a risk for the stability and progression of replication forks. Successful genome duplication in every cell cycle requires the repair or tolerance of DNA lesions, the protection of replication forks, and the ability to resume DNA synthesis after fork stalling. Failures in these processes lead to genomic instability, a hallmark of cancer and other diseases, as well as an important causal factor in aging.

Our group is interested in understanding how eukaryotic cells maintain genome stability during chromosome replication, especially under conditions that cause DNA damage or replicative stress. We study how different DNA repair, DNA damage tolerance and checkpoint proteins, in conjunction with some helicases and nucleases, facilitate chromosome replication in the presence of DNA lesions or replication perturbations. We analyse the contribution of these proteins to the integrity and function of replication forks, their regulation and their importance for cell viability under different conditions that cause DNA damage. The main aspects of these processes are evolutionarily conserved, allowing us to use the budding yeast Saccharomyces cerevisiae as a working model organism.


Figure 1. The structure-specific endonuclease Mus81-Mms4 is recruited to subnuclear foci following DNA damage or replicative stress. Cells were treated with different agents and analysed by fluorescence microscopy. Arrows indicate selected foci.


Figure 2. Schematic illustration of the processes triggering the DNA damage tolerance response. The main proteins involved in this cellular response are depicted.

If you are interested in joining our group, please write to: jatercero@cbm.csic.es


* For external calls please dial 34 91196 followed by the extension number
Last nameNameLaboratoryExt.*e-mailProfessional category
Lehmann Carl Philip4044516clehmann(at)cbm.csic.esM3
Marcos MaesoLaura de 4044516ldemarcos(at)cbm.csic.esTitulado Sup. Actividades Tecn. y Prof.GP1
Tercero OrduñaJosé Antonio4044818jatercero(at)cbm.csic.esE. Investigadores Científicos de Organismos Públicos

Relevant publications:

  • Jiménez-Martín A, Saugar I, Joseph CR, Mayer A, Lehmann CP, Szakal B, Branzei D, Tercero JA (2020) “The Mgs1/WRNIP1 ATPase is required to prevent a recombination salvage pathway at damaged replication forks”. Science Adv. 6: eaaz3327.
  • Saugar I, Jiménez-Martín A, Tercero JA (2017) "Subnuclear relocalization of structure-specific endonucleases in response to DNA damage". Cell Rep. 20: 1553-1562.
  • Morafraile EC, Diffley JFX, Tercero JA*, Segurado M* (2015) “Checkpoint-dependent RNR induction promotes fork restart after replicative stress”. Sci. Rep5: 7886.
  • Saugar I, Ortiz-Bazán MA, Tercero JA (2014) “Tolerating DNA damage during eukaryotic chromosome replication” Exp. Cell Res. 329: 170-177.
  • Ortiz-Bazán MA, Gallo-Fernández M, Saugar I, Jiménez-Martín A, Vázquez MV, Tercero JA (2014) “Rad5 plays a major role in the cellular response to DNA damage during chromosome replication”. Cell Rep. 9: 460-468.
  • Saugar I, Vázquez MV, Gallo-Fernández M, Ortiz-Bazán MA, Segurado M, Calzada A, Tercero JA (2013) “Temporal regulation of the Mus81-Mms4 endonuclease ensures cell survival under conditions of DNA damage”. Nucleic Acids Res.41: 8943-8958.
  • Gallo-Fernández M, Saugar I, Ortiz MA, Vázquez MV, Tercero, JA (2012) “Cell cycle-dependent regulation of the nuclease activity of Mus81-Eme1/Mms4”. Nucleic Acids Res. 40: 8325-8335.

Doctoral theses:

  • Alberto Jiménez Martín (2019) “La AAA+ ATPasa Mgs1/WRNIP1 y su relación con la tolerancia al daño en el DNA durante la replicación cromosómica”. Universidad Autónoma de Madrid. Supervisor: J. A. Tercero.
  • María Gallo Fernández (2017) “Regulación y función de la endonucleasa específica de estructura Mus81-Mms4/EME1”. Universidad Autónoma de Madrid. Supervisor: J. A. Tercero.
  • Mª Ángeles Ortiz Bazán (2014) “Análisis del papel de los componentes de la ruta RAD6/RAD18 de Saccharomyces cerevisiae en la tolerancia al daño en el DNA durante la replicación cromosómica”. Universidad Autónoma de Madrid. Supervisor: J. A. Tercero.
  • Mª Victoria Vázquez Sarrión (2012) “Análisis de factores implicados en la respuesta celular al daño en el DNA durante la replicación cromosómica en Saccharomyces cerevisiae. Universidad Autónoma de Madrid. Supervisor: J. A. Tercero.

NOTE! This site uses cookies and similar technologies.

If you not change browser settings, you agree to it. Learn more

I understand


What are cookies?

A cookie is a file that is downloaded to your computer when you access certain web pages. Cookies allow a web page, among other things, to store and retrieve information about the browsing habits of a user or their equipment and, depending on the information they contain and the way they use their equipment, they can be used to recognize the user.

Types of cookies

Classification of cookies is made according to a series of categories. However, it is necessary to take into account that the same cookie can be included in more than one category.

  1. Cookies according to the entity that manages them

    Depending on the entity that manages the computer or domain from which the cookies are sent and treat the data obtained, we can distinguish:

    • Own cookies: those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which the service requested by the user is provided.
    • Third party cookies: those that are sent to the user's terminal equipment from a computer or domain that is not managed by the publisher, but by another entity that processes the data obtained through the cookies. When cookies are installed from a computer or domain managed by the publisher itself, but the information collected through them is managed by a third party, they cannot be considered as own cookies.

  2. Cookies according to the period of time they remain activated

    Depending on the length of time that they remain activated in the terminal equipment, we can distinguish:

    • Session cookies: type of cookies designed to collect and store data while the user accesses a web page. They are usually used to store information that only is kept to provide the service requested by the user on a single occasion (e.g. a list of products purchased).
    • Persistent cookies: type of cookies in which the data is still stored in the terminal and can be accessed and processed during a period defined by the person responsible for the cookie, which can range from a few minutes to several years.

  3. Cookies according to their purpose

    Depending on the purpose for which the data obtained through cookies are processed, we can distinguish between:

    • Technical cookies: those that allow the user to navigate through a web page, platform or application and the use of different options or services that exist in it, such as controlling traffic and data communication, identifying the session, access to restricted access parts, remember the elements that make up an order, perform the purchase process of an order, make a registration or participation in an event, use security elements during navigation, store content for the broadcast videos or sound or share content through social networks.
    • Personalization cookies: those that allow the user to access the service with some predefined general characteristics based on a series of criteria in the user's terminal, such as the language, the type of browser through which the user accesses the service, the regional configuration from where you access the service, etc.
    • Analytical cookies: those that allow the person responsible for them to monitor and analyse the behaviour of the users of the websites to which they are linked. The information collected through this type of cookies is used in the measurement of the activity of the websites, applications or platforms, and for the elaboration of navigation profiles of the users of said sites, applications and platforms, in order to introduce improvements in the analysis of the data of use made by the users of the service.

Cookies used on our website

The CBMSO website uses Google Analytics. Google Analytics is a simple and easy to use tool that helps website owners to measure how users interact with the content of the site. You can consult more information about the cookies used by Google Analitycs in this link.

Acceptance of the Cookies Policy

The CBMSO assumes that you accept the use of cookies if you continue browsing, considering that it is a conscious and positive action from which the user's consent is inferred. In this regard, you are previously informed that such behaviour will be interpreted that you accept the installation and use of cookies.

Knowing this information, it is possible to carry out the following actions:

  • Accept cookies: if the user presses the acceptance button, this warning will not be displayed again when accessing any page of the portal.
  • Review the cookies policy: the user can access to this page in which the use of cookies is detailed, as well as links to modify the browser settings.

How to modify the configuration of cookies

Using your browser you can restrict, block or delete cookies from any web page. In each browser the process is different, here we show you links on this particular of the most used browsers: