CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOACaptura de pantalla 2022 09 14 a las 10.27.10    

Confocal microscopy

Latest news

  • 2022 Rates in effect for the SMOC from (01-03-2022)
Scientific supervisor: Francisco Javier Díez-Guerra
Technical director: Ángeles Muñoz

  confocal-cbm (at) listas.csic.es
  Third floor - 310

The optical and confocal microscopy facility (SMOC) is the responsible of maintaining and managing the advanced-optical-microscopy equipment in the CBMSO. It also offers support, advice and training in optical microscopy techniques and image analysis, it distributes reagents and other materials related to microscopy, and is responsible for finding resources to acquire new equipment that suits the requests of CBM researchers.

Our Facility, as a central scientific service at the Centro de Biología Molecular "Severo Ochoa", does not carry out any scientific research work but offers:

  • 7 confocal microscopy systems and 6 wide-field microscopes, with different levels of automation, allowing studies on live and fixed samples. In addition, 2 workstations for image analysis, a vibratome and a stereomicroscope.
  • An online platform to schedule and manage the equipment and invoicing.
  • A stock of reagents and materials useful for microscopy.
  • A quality management system ISO 9001-2015 certified by AENOR, since March 2009.



  • "Setting up and running an Advanced Light Microscopy and Imaging Facility". Carlos Sánchez, Mª Ángeles Muñoz, Maite Villalba, Verónica Labrador and F. Javier Díez-Guerra 2010. Current Protocols in Cytometry. 57:12.22.1-12.22.21.
  • "Técnicas avanzadas de microscopía óptica para el estudio de la fisiopatología celular". Francisco Javier Díez Guerra y Carlos Sánchez Martín. Capítulo 5, Sección II (Papel de la Biología Molecular y Celular en el estudio de las Enfermedades Renales). Nefrología Clínica. (4ª Edición. Noviembre 2013). Autor: Manuel Arias Rodríguez. Editor: L. Hernando Avendaño. Editorial Médica Panamericana

* For external calls please dial 34 91196 followed by the extension number
Last name Name Laboratory Ext.* Schedule e-mail Professional category
Calvo Cazalilla Elena 310 4643 9:00 - 16:30 elena.calvo@cbm.csic.es Tco. Sup.Investig. y Laboratorio, GP3
Gallego García Carlos 310 4643 8:00 - 15:30 cgallego(at)cbm.csic.es Tit.Sup.Activ.Técn.y Profes. GP1
Muñoz Alcalá Mª Angeles 310 4643/4613 9:00 - 16:30 mamunoz(at)cbm.csic.es E.Ayudantes De Invest. De Los Oo.Publicos De Investigacion
Sánchez Jiménez Carmen 310 4643 9:30-17:00 csjimenez(at)cbm.csic.es Titulado Sup.de Actividades Técn. y Profes. GP1
Vega Sabugo Francisco José 310 4643 9:30 - 17:00 fjvega(at)cbm.csic.es Titulado Sup. Actividades Tecn. y Prof.GP1
Villalba Villacorta María Teresa 310 4643 8:00 - 15:30 tvillalba(at)cbm.csic.es Ayudante Investigación
STED & FLIM Microscope
Available reagents at the Confocal Microscopy Facility
General recommendations - Tips
Fluorophores and fluorescent proteins
  • Check the excitation and emission spectra of the florophores and fluorescent proteins in order to optimize the method used for imaging.
  • When a fluorescent protein (FP) is used for the first time check the emission spectrum, by spectral detection, to ensure the real identity of the protein.
  • The laser line 405 nm may photoconvert your FP (change the emission profile). Check it.

Servicio de Microscopía Optica y Confocal (Lab.310)

Centro de Biología Molecular "Severo Ochoa" (CSIC-UAM)

C/Nicolás Cabrera,1

Universidad Autónoma de Madrid. Cantoblanco.

28049. Madrid


Despacho: 91 196 4613

Laboratorio 310: 91 196 4643

Laboratorio 336: 91 196 4660

Fax. 91 196 4420

E-mail: confocal-cbm@listas.csic.es

SMOC en 3 plantab 1

Transporte en la Comunidad de Madrid

Transporte en la U. Autónoma de Madrid


Líneas C7, C8 y C10 de Cercanías: Parla-Atocha-Chamartín-Cantoblanco-Alcobendas/S.S. de los Reyes o Colmenar Viejo.

Estación de Cantoblanco.


Líneas 714, 827, 827B y 828


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