CENTRO DE BIOLOGÍA MOLECULAR SEVERO OCHOACaptura de pantalla 2022 09 14 a las 10.27.10    

Drosophila transgenesis

Scientific supervisor: Carlos Estella
Technical directors: Mar Casado | Nuria Esteban
  transgenesisdro (at) cbm.csic.es
  Fourth floor - 423A


The Drosophila Transgenesis Facility was created in 2008 within the Consolider Project “From Genes to Shape” with the aim of bringing this basic and crucial technique to the Consolider members in a simple and cost effective way.

The facility success during the initial founding years prompted several groups that did not belong to the consortium to show an interest in our services. Thus, it was decided to extend it to all potential users in Spain and also abroad.

The facility has significantly evolved since its origins, adding most transgenesis techniques to our portfolio. Currently, our team is composed of three highly qualified professionals with more than ten years of experience in transgenesis of Drosophila. We offer the following services:

  • Classic transgenesis by P-element mediated random insertion of DNA.
  • Targeted transgenesis mediated by PhiC31 integrase using both, plasmids and BACs.
  • Injection of DNA cocktails for CRISPR/Cas9 aided genome editing.

The keys to our success are:

  • Efficiency: We have already injected more than 2000 DNA constructs with an overall success of about 98%.
  • Speed: We deliver the injected larvae just 5 to 9 days after reception of a new DNA sample.
  • Low cost: Very competitive pricing compared to other national and international similar facilities.

We currently provide our services to more than 20 groups in Spain and 10 groups in Great Britain, Germany, France and Portugal.


* For external calls please dial 34 91196 followed by the extension number
Last nameNameLaboratoryExt.*e-mail

We provide the following SERVICES:

  1. Classic transgenesis by P-element mediated random insertion of DNA:
    The relevant DNA fragment should be cloned into a plasmid containing P-element inverted repeats for integration and a mini-white gene to screen for positives.
    About 10 micrograms of DNA are needed in a maximum volume of 100 microliters.
    Please, provide only high quality DNA preps (Qiagen kit or similar) in TE buffer or water. There is no need for you to include the helper DNA.

  2. Targeted transgenesis mediated by PhiC31 integrase:
    This technique allows the insertion of transgenes into well-defined docking sites available at several chromosomal locations. You can find information about this methodology for example in this paper and also in FlyC31.

    Transgenes should be cloned into attB containing plasmids that also carry a mini-white or a vermilion gene for identification of positives. We require high quality DNA preps obtained with the Qiagen kit or similar. Dissolve the DNA in water at 300 nanograms/microliter and a minimum volume of 10 microliters.

    We routinely use the following attP docking sites. However, contact us in case you have other requirements:

    / yw background:
        -M{3xP3-RFP.attP'}ZH-22A: Insertion at 2L-22A
        -M{3xP3-RFP.attP}ZH-51D: Insertion at 2R-51D
        -M{3xP3-RFP.attP'}ZH-68E: Insertion at 3L-68E
        -M{3xP3-RFP.attP}ZH-86Fb: Insertion at 3R-86F

    v background (required to generate stable lines for some CRISPR/Cas9 applications):
        -P{CaryP}attP40: Insertion at 2L-25C7
        -P{CaryP}attP2: Insertion at 3L-68A4

  3. Injection of nucleic acid cocktails for CRISPR/Cas9 aided genome editing:
    We can inject nucleic acid mixtures containing gRNAs, gRNA coding plasmids, targeting vectors and ssDNA templates for CRISPR/Cas9 aided genome editing. The recipient strain we use expresses Cas9 under the nanos promoter (see table below). Unfortunately, we cannot provide support regarding experimental design or the multiple available technical approaches. The user should provide the nucleic acid mixture ready to be injected. The number of injected embryos should also be defined by the user. In our experience, about 500 embryos are needed for a knock-in experiment.

We recommend Qiagen plasmid purification kit or similar for DNA purification. DNA must be quantified by the user before shipment.

For P-element or PhiC31 mediated transgenesis, we inject 150-200 embryos for each construct. Surviving larvae (50-100) are shipped 3-4 days after injection and the user is responsible to screen for positives. In case no transformants are obtained, we will inject the sample a second time with priority and free of charge.

Send your samples to the following address:

Eva Caminero
Centro de Biología Molecular S.O.
C/Nicolás Cabrera 1
Teléfono: +34-911964401

Please, also include your instructions using this form. If this is your first time using the facility, you should also fill some legal paperwork.

Each DNA construct injected:

-CBM/UAM users: 40€
-CSIC users: 46€
-Other users: 120€


Total DNA: 10µg





2L y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP'}ZH-22A


2R y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP}ZH-51D


3L y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP'}ZH-68E


3R y[1] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP}ZH-86Fb


25C y[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X;P{y[+t7.7]=CaryP}attP40


68A y[1] sc[1] v[1] P{y[+t7.7]=nos-phiC31\int.NLS}X; P{y[+t7.7]=CaryP}attP2



Noscas9 y[1] M{w[+mC]=nos-Cas9.P}ZH-2A w[*]


  y[1] M{GFP[E.3xP3]=vas-Cas9.RFP-}ZH-2A w[1118]  B#55821

Drosophila Transgenesis Facility

Centro de Biología Molecular Severo Ochoa (CSIC-UAM)

C/ Nicolás Cabrera,1

Universidad Autónoma de Madrid. Cantoblanco.

28049 Madrid

Phone: 911 964 610

Laboratory: 423A

E-mail: transgenesisdro@cbm.csic.es

NOTE! This site uses cookies and similar technologies.

If you not change browser settings, you agree to it. Learn more

I understand


What are cookies?

A cookie is a file that is downloaded to your computer when you access certain web pages. Cookies allow a web page, among other things, to store and retrieve information about the browsing habits of a user or their equipment and, depending on the information they contain and the way they use their equipment, they can be used to recognize the user.

Types of cookies

Classification of cookies is made according to a series of categories. However, it is necessary to take into account that the same cookie can be included in more than one category.

  1. Cookies according to the entity that manages them

    Depending on the entity that manages the computer or domain from which the cookies are sent and treat the data obtained, we can distinguish:

    • Own cookies: those that are sent to the user's terminal equipment from a computer or domain managed by the editor itself and from which the service requested by the user is provided.
    • Third party cookies: those that are sent to the user's terminal equipment from a computer or domain that is not managed by the publisher, but by another entity that processes the data obtained through the cookies. When cookies are installed from a computer or domain managed by the publisher itself, but the information collected through them is managed by a third party, they cannot be considered as own cookies.

  2. Cookies according to the period of time they remain activated

    Depending on the length of time that they remain activated in the terminal equipment, we can distinguish:

    • Session cookies: type of cookies designed to collect and store data while the user accesses a web page. They are usually used to store information that only is kept to provide the service requested by the user on a single occasion (e.g. a list of products purchased).
    • Persistent cookies: type of cookies in which the data is still stored in the terminal and can be accessed and processed during a period defined by the person responsible for the cookie, which can range from a few minutes to several years.

  3. Cookies according to their purpose

    Depending on the purpose for which the data obtained through cookies are processed, we can distinguish between:

    • Technical cookies: those that allow the user to navigate through a web page, platform or application and the use of different options or services that exist in it, such as controlling traffic and data communication, identifying the session, access to restricted access parts, remember the elements that make up an order, perform the purchase process of an order, make a registration or participation in an event, use security elements during navigation, store content for the broadcast videos or sound or share content through social networks.
    • Personalization cookies: those that allow the user to access the service with some predefined general characteristics based on a series of criteria in the user's terminal, such as the language, the type of browser through which the user accesses the service, the regional configuration from where you access the service, etc.
    • Analytical cookies: those that allow the person responsible for them to monitor and analyse the behaviour of the users of the websites to which they are linked. The information collected through this type of cookies is used in the measurement of the activity of the websites, applications or platforms, and for the elaboration of navigation profiles of the users of said sites, applications and platforms, in order to introduce improvements in the analysis of the data of use made by the users of the service.

Cookies used on our website

The CBMSO website uses Google Analytics. Google Analytics is a simple and easy to use tool that helps website owners to measure how users interact with the content of the site. You can consult more information about the cookies used by Google Analitycs in this link.

Acceptance of the Cookies Policy

The CBMSO assumes that you accept the use of cookies if you continue browsing, considering that it is a conscious and positive action from which the user's consent is inferred. In this regard, you are previously informed that such behaviour will be interpreted that you accept the installation and use of cookies.

Knowing this information, it is possible to carry out the following actions:

  • Accept cookies: if the user presses the acceptance button, this warning will not be displayed again when accessing any page of the portal.
  • Review the cookies policy: the user can access to this page in which the use of cookies is detailed, as well as links to modify the browser settings.

How to modify the configuration of cookies

Using your browser you can restrict, block or delete cookies from any web page. In each browser the process is different, here we show you links on this particular of the most used browsers: